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题名: Investigation of alginate-epsilon-poly-L-lysine microcapsules for cell microencapsulation
作者: Ma, Ying1, 2;  Zhang, Ying1;  Liu, Yang1;  Chen, Li1, 2;  Li, Shen1, 2;  Zhao, Wei1, 2;  Sun, Guangwei1;  Li, Na1, 2;  Wang, Yu1, 2;  Guo, Xin1;  Lv, Guojun1;  Ma, Xiaojun1
关键词: microencapsulation ;  alginate ;  poly-amino acid membrane ;  E-poly-L-lysine ;  cell culture
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2013-05-01
DOI: 10.1002/jbm.a.34418
卷: 101, 期:5, 页:1265-1273
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: Cell microencapsulation is a promising approach for cell implantation, cell-based gene therapy, and large-scale cell culture. The well-studied -AP (alginate-poly-L-lysine) microcapsules have been restricted to large-scale cell-culture and clinical applications because of high costs and cytotoxic effects in some cases. This study used E-poly-L-lysine (E-PLL), a high-biocompatible and low-cost food additives produced by fermentation, to prepare E-AP (alginateE-PLL) microcapsules with various thickness membranes and swelling behaviors. E-AP microcapsules were permeable to BSA, a standard protein of culture medium. E-AP-microencapsulated Chinese hamster ovary (CHO) cells proliferated with culture time; no obvious difference with -AP-microencapsulated CHO cells during the early 19 days. Whereas E-AP-microencapsulated CHO cells kept higher viability (OD = 0.646 +/- 0.012) on the 22nd day and microcapsule strength (integrity rate of 88%) on the 24th day than that of -AP microcapsules (OD = 0.558 +/- 0.025, integrity rate of 80%). E-AP (alginate-E-PLL) microcapsules exhibited more superior properties and could lower the costs to broaden the applications of microencapsulation technology. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
关键词[WOS]: MONOCLONAL-ANTIBODY PRODUCTION ;  POLYCATION MICROCAPSULES ;  ANIMAL-CELLS ;  STABILITY ;  GROWTH ;  PERMEABILITY ;  OPTIMIZATION ;  TECHNOLOGY ;  DELIVERY ;  CHITOSAN
语种: 英语
WOS记录号: WOS:000316815700005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137670
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Ma, Ying,Zhang, Ying,Liu, Yang,et al. Investigation of alginate-epsilon-poly-L-lysine microcapsules for cell microencapsulation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2013,101(5):1265-1273.
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