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Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography
Shen, Aijin1; Li, Xiuling1; Dong, Xuefang1; Wei, Jie2; Guo, Zhimou1; Liang, Xinmiao1,2
KeywordHydrophilic Interaction/cation-exchange Chromatography (Hilic/cex) Glutathione Thiol-ene Zwitterionic Stationary Phase Mixed-mode
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2013-11-01
DOI10.1016/j.chroma.2013.09.002
Volume1314Pages:63-69
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordINTERACTION LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY MASS-SPECTROMETRY ; POLAR COMPOUNDS ; REVERSED-PHASE ; CLICK CHEMISTRY ; DRUG DISCOVERY ; SEPARATION ; PEPTIDES ; STRATEGY ; HISTONES
AbstractAs a naturally hydrophilic peptide, glutathione was facilely immobilized onto silica surface to obtain a novel hydrophilic interaction/cation-exchange mixed-mode chromatographic stationary phase (Click TE-GSH) via copper-free "thiol-ene" click chemistry. The resulting material was characterized by solid state C-13/CP MAS NMR and elemental analysis. The measurement of zeta-potential indicated the cation-exchange characteristics and adjustable surface charge density of Click TE-GSH material. The influence of acetonitrile content and pH value on the retention of ionic compounds was investigated for understanding the chromatographic behaviors. The results demonstrated that Click TE-GSH column could provide both hydrophilic and cation-exchange interaction. Taking advantage of the good hydrophilicity and inherent cation-exchange characteristics of Click TE-GSH material, the resolution of neutral fructosan with high degree of polymerization (DP), basic chitooligosaccharides and strongly acidic carrageenan oligosaccharides was successfully realized in hydrophilic interaction chromatography (HILIC), hydrophilic interaction/cation-exchange mixed-mode chromatography (HILIC/CEX), cation-exchange chromatography (CEX) and electrostatic repulsion/hydrophilic interaction chromatography (ERLIC). On the other hand, the separation of standard peptides varying in hydrophobicity/hydrophilicity and charge was achieved in both CEX and HILIC/CEX mode with high efficiency and distinct selectivity. To further demonstrate the versatility and applicability of Click TE-GSH stationary phase, the separation of a human serum albumin (HSA) tryptic digest was performed in HILIC/CEX mode. Peptides were adequately resolved and up to 86 HSA peptides were identified with sequence coverage of 85%. The results indicated the good potential of Click TE-GSH material in glycomics and proteomics. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000325841300009
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137685
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Beijing 100864, Peoples R China
2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
Recommended Citation
GB/T 7714
Shen, Aijin,Li, Xiuling,Dong, Xuefang,et al. Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1314:63-69.
APA Shen, Aijin,Li, Xiuling,Dong, Xuefang,Wei, Jie,Guo, Zhimou,&Liang, Xinmiao.(2013).Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography.JOURNAL OF CHROMATOGRAPHY A,1314,63-69.
MLA Shen, Aijin,et al."Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography".JOURNAL OF CHROMATOGRAPHY A 1314(2013):63-69.
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