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题名: Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material
作者: Chen, Linlin1;  Dong, Xuefang2;  Cao, Liwei2;  Guo, Zhimou2;  Yu, Long2;  Zou, Lijuan1;  Liang, Xinmiao2
刊名: ANALYTICAL METHODS
发表日期: 2013
DOI: 10.1039/c3ay41590b
卷: 5, 期:24, 页:6919-6924
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Chemistry, Analytical ;  Food Science & Technology ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Food Science & Technology ;  Spectroscopy
英文摘要: Protein glycosylation analysis based on mass spectrometry (MS) remains a major analytical challenge, because of the low proportions of glycopeptides in the glycoprotein digest and the suppression effect of the coexisting non-glycosylated peptides. Therefore, selective enrichment of glycopeptides prior to MS is of great significance. In this study, we presented hydrophilic interaction/cation-exchange chromatography (HILIC/CEX) for glycopeptide enrichment by applying a customized material named MEX. MEX is a silica-based material bonded with two types of functional groups, benzenesulfonic acid and cyano-propyl groups. Compared with traditional strong-cation exchange (SCX) columns, moderate retention of analytes can be obtained on MEX due to the decreased ion-exchange capacity caused by the introduction of the cyano-propyl groups. Meanwhile, superior HILIC selectivity was also exhibited on MEX. In glycosylation analysis, MEX exhibited high selectivity and broad coverage for glycopeptides, which indicated the promising potential of the MEX material in glycosylation analysis.
关键词[WOS]: PHASE LIQUID-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  N-GLYCANS ;  GLYCOSYLATION HETEROGENEITY ;  SELECTIVE ENRICHMENT ;  CANCER-PATIENTS ;  GLYCOPROTEINS ;  IDENTIFICATION ;  SEPARATION ;  PROTEINS
语种: 英语
WOS记录号: WOS:000327506600006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137699
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Affiliated Hosp 2, Dept Radiol, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Chen, Linlin,Dong, Xuefang,Cao, Liwei,et al. Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material[J]. ANALYTICAL METHODS,2013,5(24):6919-6924.
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