DICP OpenIR
Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material
Chen, Linlin1; Dong, Xuefang2; Cao, Liwei2; Guo, Zhimou2; Yu, Long2; Zou, Lijuan1; Liang, Xinmiao2
Source PublicationANALYTICAL METHODS
2013
DOI10.1039/c3ay41590b
Volume5Issue:24Pages:6919-6924
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS KeywordPHASE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; N-GLYCANS ; GLYCOSYLATION HETEROGENEITY ; SELECTIVE ENRICHMENT ; CANCER-PATIENTS ; GLYCOPROTEINS ; IDENTIFICATION ; SEPARATION ; PROTEINS
AbstractProtein glycosylation analysis based on mass spectrometry (MS) remains a major analytical challenge, because of the low proportions of glycopeptides in the glycoprotein digest and the suppression effect of the coexisting non-glycosylated peptides. Therefore, selective enrichment of glycopeptides prior to MS is of great significance. In this study, we presented hydrophilic interaction/cation-exchange chromatography (HILIC/CEX) for glycopeptide enrichment by applying a customized material named MEX. MEX is a silica-based material bonded with two types of functional groups, benzenesulfonic acid and cyano-propyl groups. Compared with traditional strong-cation exchange (SCX) columns, moderate retention of analytes can be obtained on MEX due to the decreased ion-exchange capacity caused by the introduction of the cyano-propyl groups. Meanwhile, superior HILIC selectivity was also exhibited on MEX. In glycosylation analysis, MEX exhibited high selectivity and broad coverage for glycopeptides, which indicated the promising potential of the MEX material in glycosylation analysis.
Language英语
WOS IDWOS:000327506600006
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137699
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Med Univ, Affiliated Hosp 2, Dept Radiol, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Chen, Linlin,Dong, Xuefang,Cao, Liwei,et al. Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material[J]. ANALYTICAL METHODS,2013,5(24):6919-6924.
APA Chen, Linlin.,Dong, Xuefang.,Cao, Liwei.,Guo, Zhimou.,Yu, Long.,...&Liang, Xinmiao.(2013).Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material.ANALYTICAL METHODS,5(24),6919-6924.
MLA Chen, Linlin,et al."Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material".ANALYTICAL METHODS 5.24(2013):6919-6924.
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