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题名: Sequence characterization and computational analysis of the non-ribosomal peptide synthetases controlling biosynthesis of lipopeptides, fengycins and bacillomycin D, from Bacillus amyloliquefaciens Q-426
作者: Zhao, Pengchao1, 2;  Quan, Chunshan3;  Jin, Liming3;  Wang, Lina1, 2;  Guo, Xinjuan1, 2;  Fan, Shengdi3
关键词: Bacillomycin ;  Bacillus amyloliquefaciens ;  Bioinformatics analysis ;  Fengycin ;  Functional domains ;  Iturin ;  Non-ribosomal peptide synthetases ;  Sequence analysis
刊名: BIOTECHNOLOGY LETTERS
发表日期: 2013-12-01
DOI: 10.1007/s10529-013-1320-5
卷: 35, 期:12, 页:2155-2163
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Lipopeptides secreted by bacteria attract interest because of their uses in biomedicine, biotechnology and food technology; however, harnessing their megasynthases (non-ribosomal peptide synthetases, NRPSs) has met with some difficulties in heterologous expression and crystallization. Here, we used similarity and phylogenetic analysis of NRPS sequences, including the fengycin and iturin family synthetases from Bacillus spp., and have developed a novel approach for delineating the length and boundaries of NRPS domains from Bacillus amyloliquefaciens strain Q-426. The sequences were further characterized (including specific residues and conserved motifs) that gave insight into the basis of the substrate specificity. Data from the prediction of the NRPS domains, obtained by the self-optimized prediction method with Alignment program, showed they are all structurally unstable, making it difficult to determine their crystal structures.
关键词[WOS]: THIOESTERASE DOMAIN ;  ADENYLATION DOMAINS ;  STRUCTURAL BASIS ;  GRAMICIDIN-S ;  SURFACTIN ;  EVOLUTION ;  RECOGNITION ;  CYCLIZATION ;  DAPTOMYCIN ;  PREDICTION
语种: 英语
WOS记录号: WOS:000326631100025
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137708
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Nationalities Univ, Dept Life Sci, Dalian 116600, Peoples R China

Recommended Citation:
Zhao, Pengchao,Quan, Chunshan,Jin, Liming,et al. Sequence characterization and computational analysis of the non-ribosomal peptide synthetases controlling biosynthesis of lipopeptides, fengycins and bacillomycin D, from Bacillus amyloliquefaciens Q-426[J]. BIOTECHNOLOGY LETTERS,2013,35(12):2155-2163.
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