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Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel
Yu, Xiaoming1,2; Zhao, Peng2; Zhang, Lihua2; Zhang, Yukui2
关键词Target Proteins Screening Polyacrylamide Cryogel Monolith Phage-displayed Cdna Libary Biopanning
刊名BIOMEDICAL CHROMATOGRAPHY
2013-11-01
DOI10.1002/bmc.2962
27期:11页:1574-1579
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]DNA METHYLTRANSFERASES ; AFFINITY LIGANDS ; BINDING ; PROTEIN ; IDENTIFICATION ; PURIFICATION ; ADSORBENTS ; CHROMATOGRAPHY ; EXCHANGE ; CAPTURE
英文摘要Monolithic polyacrylamide cryogel was prepared and utilized as a new matrix for drug immobilization to screen against phage-displayed human liver cDNA library. The macropores and hydrophilic nature of the cryogel made it possible for phage particles to pass unhindered. Doxorubicin, an anticancer drug, was covalently bonded to the monolithic cryogel by the glutaraldehyde method, and after five rounds of affinity selection performed in an SPE cartridge, phage clones that displayed Homo sapiens methyl CpG binding protein 2 (MeCP2) were selectively enriched. The interaction between doxorubicin and MeCP2 displayed phages was further validated by studying the retention of doxorubicin on MeCP2 phage-coupled cryogel. These results demonstrate that drug-coupled polyacrylamide cryogel might be a promising kind of matrix for screening target proteins against phage-displayed library. Copyright (c) 2013 John Wiley & Sons, Ltd.
语种英语
WOS记录号WOS:000328324100028
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137722
专题中国科学院大连化学物理研究所
作者单位1.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Yu, Xiaoming,Zhao, Peng,Zhang, Lihua,et al. Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel[J]. BIOMEDICAL CHROMATOGRAPHY,2013,27(11):1574-1579.
APA Yu, Xiaoming,Zhao, Peng,Zhang, Lihua,&Zhang, Yukui.(2013).Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel.BIOMEDICAL CHROMATOGRAPHY,27(11),1574-1579.
MLA Yu, Xiaoming,et al."Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel".BIOMEDICAL CHROMATOGRAPHY 27.11(2013):1574-1579.
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