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题名: Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel
作者: Yu, Xiaoming1, 2;  Zhao, Peng2;  Zhang, Lihua2;  Zhang, Yukui2
关键词: target proteins screening ;  polyacrylamide cryogel ;  monolith ;  phage-displayed cDNA libary ;  biopanning
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2013-11-01
DOI: 10.1002/bmc.2962
卷: 27, 期:11, 页:1574-1579
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Pharmacology & Pharmacy
英文摘要: Monolithic polyacrylamide cryogel was prepared and utilized as a new matrix for drug immobilization to screen against phage-displayed human liver cDNA library. The macropores and hydrophilic nature of the cryogel made it possible for phage particles to pass unhindered. Doxorubicin, an anticancer drug, was covalently bonded to the monolithic cryogel by the glutaraldehyde method, and after five rounds of affinity selection performed in an SPE cartridge, phage clones that displayed Homo sapiens methyl CpG binding protein 2 (MeCP2) were selectively enriched. The interaction between doxorubicin and MeCP2 displayed phages was further validated by studying the retention of doxorubicin on MeCP2 phage-coupled cryogel. These results demonstrate that drug-coupled polyacrylamide cryogel might be a promising kind of matrix for screening target proteins against phage-displayed library. Copyright (c) 2013 John Wiley & Sons, Ltd.
关键词[WOS]: DNA METHYLTRANSFERASES ;  AFFINITY LIGANDS ;  BINDING ;  PROTEIN ;  IDENTIFICATION ;  PURIFICATION ;  ADSORBENTS ;  CHROMATOGRAPHY ;  EXCHANGE ;  CAPTURE
语种: 英语
WOS记录号: WOS:000328324100028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137722
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Yu, Xiaoming,Zhao, Peng,Zhang, Lihua,et al. Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel[J]. BIOMEDICAL CHROMATOGRAPHY,2013,27(11):1574-1579.
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