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Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry
Chen, Shili1; Hoene, Miriam2,3; Li, Jia1; Li, Yanjie1; Zhao, Xinjie1; Haering, Hans-Ulrich3,4; Schleicher, Erwin D.2,3; Weigert, Cora2,3; Xu, Guowang1; Lehmann, Rainer2,3
关键词Liquid Chromatography/mass Spectrometry Metabolic Profiling Sample Pretreatment Methyl Tert-butyl Ether (Mtbe) Biopsy Metabolomics
刊名JOURNAL OF CHROMATOGRAPHY A
2013-07-12
DOI10.1016/j.chroma.2013.05.019
1298页:9-16
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]FATTY-ACID-COMPOSITION ; MASS-SPECTROMETRY ; MUSCLE ; RESISTANCE ; DISEASE ; PLASMA ; BRAIN ; LIVER ; SERUM ; MODEL
英文摘要A common challenge for scientists working with animal tissue or human biopsy samples is the limitation of material and consequently, the difficulty to perform comprehensive metabolic profiling within one experiment. Here, we present a novel approach to simultaneously perform targeted and non-targeted metabolomics as well as lipidomics from one small piece of liver or muscle tissue by ultra-high performance liquid chromatography/mass spectrometry (UHPLC/MS) following a methyl tert-butyl ether (MTBE)-based extraction. Equal relative amounts of the resulting polar and non-polar fractions were pooled, evaporated and reconstituted in the appropriate solvent for UHPLC/MS analysis. This mix was comparable or superior in yield and reproducibility to a standard 80% methanol extraction for the profiling of polar and lipophilic metabolites (free carnitine, acylcarnitines and FFA). The mix was also suitable for non-targeted metabolomics, an easy measure to increase the metabolite coverage by 30% relative to using the polar fraction alone. Lipidomics was performed from an aliquot of the non-polar fraction. This novel strategy could successfully be applied to one mouse soleus muscle with a dry weight of merely 2.5 mg. By enabling a simultaneous profiling of lipids and metabolites with mixed polarity while saving material for molecular, biochemical or histological analyses, our approach may open up new perspectives toward a comprehensive investigation of small, valuable tissue samples. (C) 2013 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000321174700002
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被引频次:70[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137725
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Hosp Tuebingen, Dept Internal Med 4, Div Clin Chem & Pathobiochem, Tubingen, Germany
3.Univ Tubingen, Helmholtz Ctr Munich, Inst Diabet Res & Metab Dis, Paul Langerhans Inst Tuebingen, Tubingen, Germany
4.Univ Hosp Tuebingen, Dept Internal Med 4, Div Endocrinol Diabetol Vasc Med Nephrol & Clin C, Tubingen, Germany
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Chen, Shili,Hoene, Miriam,Li, Jia,et al. Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1298:9-16.
APA Chen, Shili.,Hoene, Miriam.,Li, Jia.,Li, Yanjie.,Zhao, Xinjie.,...&Lehmann, Rainer.(2013).Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1298,9-16.
MLA Chen, Shili,et al."Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1298(2013):9-16.
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