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题名: The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells
作者: Zhang, Qiaoyin1;  Huang, Nianyu1;  Wang, Junzhi1, 2;  Luo, Huajun1;  He, Haibo1;  Ding, Mingruo1;  Deng, Wei-Qiao3;  Zou, Kun1
关键词: Trametenolic acid B ;  H+/K+-ATPase inhibitory activity ;  Docking simulation ;  Anti-gastric cancer
刊名: FITOTERAPIA
发表日期: 2013-09-01
DOI: 10.1016/j.fitote.2013.05.021
卷: 89, 页:210-217
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Medicinal ;  Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
英文摘要: Trametenolic acid B (TAB), the bioactive component in the Trametes lactinea (Berk.) Pat, was reported to possess cytotoxic activities and thrombin inhibiting effects. This study was performed to investigate the effects of TAB on H+/K+-ATPase and gastric cancer. The H+/K+-ATPase inhibitory activity was determined by gastric parietal cells. Compared to the normal control group, TAB (10, 20, 40 and 80 ug/mL) inhibited the H+/K+-ATPase activity by 15.97, 16.96, 24.86 and 16.25%, respectively. In the study, 36 Kunming mice were randomly divided into six groups: control, model, TAB-L (TAB, 5 mg/kg/day, i.g.), TAB-M (TAB, 20 mg/kg/day, i.g.), TAB-H (TAB, 40 mg/kg/day, i.g.) and omeprazole (OL, 10 mg/kg/day, i.g.). All mice except the control group were administrated with anhydrous alcohol (5.0 mL/kg, i.g.) for induced gastric-ulcer 1 h after the 5th day. At the same time, the control mice were given the same volume of physiological saline. After 4 h, TAB was evaluated for H+/K+-ATPase inhibitory activities of ulcerative gaster, gastric ulcer index and ulcer inhibition. In vitro, the anti-proliferation effect of TAB to gastric cancer cell (HGC-27) in acid environment was detected by MTT, and the apoptosis morphological changes were also observed by Hoechst 33258 dye assay. The results indicated that TAB inhibited moderately H+/K+-ATPase activity in vitro. Compared to the model group, TAB showed anti-ulcer effects in gastric tissue with the dosages of 20 and 5 mg/kg in vivo. Apart from that, TAB could selectively inhibit gastric cancer cell viability and reduce cell apoptosis against HGC-27 cells at low doses in acid environment. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
关键词[WOS]: PROTON PUMP INHIBITOR ;  PEPTIC-ULCER DISEASE ;  HELICOBACTER-PYLORI ;  INONOTUS-OBLIQUUS ;  IN-VITRO ;  H+,K+-ATPASE ;  TRITERPENES ;  APOPTOSIS ;  VIVO
语种: 英语
WOS记录号: WOS:000323856500024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137755
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.China Three Gorges Univ, Hubei Key Lab Nat Prod Res & Dev, Yichang 443002, Peoples R China
2.Hubei Tujia Inst Med, Yichang 443002, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Qiaoyin,Huang, Nianyu,Wang, Junzhi,et al. The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells[J]. FITOTERAPIA,2013,89:210-217.
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