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Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography
Ding Ling; Dong Jun; Xiao Yuan-Sheng; Zhang Xiu-Li; Xue Xing-Ya; Liang Xin-Miao
关键词Protein Reversed Phase Liquid Chromatography Retention Equation Predict Optimization
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2013-02-01
DOI10.3724/SP.J.1096.2013.20881
41期:2页:181-186
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]HYDROPHOBIC INTERACTION CHROMATOGRAPHY ; AFFINITY-CHROMATOGRAPHY ; PURIFICATION ; SEPARATION ; GRADIENT ; SYSTEMS ; ELUTION
英文摘要Study on the retention equation for protein in RPLC can contribute to optimizing gradient conditions for protein separation. In this study, five standard proteins, insulin, cytochrome C, lysozyme, transferrin and myoglobin were selected, and their retention time on three reversed-phase columns (Waters Symmetry 300 C-4, Waters Symmetry 300 C-18, C8HC) under six different linear gradients was obtained. According to nonlinear curve fit (CSASS software), the retention equation for five standard proteins on three columns was established. The regression coefficients of the 15 equations were all above 0: 999, indicating that the equation could accurately describe protein retention behavior on RPLC mode. Based on the equation, retention time for 5 standard proteins under other gradient conditions was predicted, and the relative errors for each protein were below 5%. This retention equation could be used in optimizing gradient conditions for protein separation on RPLC, leading to rapid baseline separation of five mixed standard proteins within 20 min.
语种英语
WOS记录号WOS:000318392300004
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/137791
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Ding Ling,Dong Jun,Xiao Yuan-Sheng,et al. Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(2):181-186.
APA Ding Ling,Dong Jun,Xiao Yuan-Sheng,Zhang Xiu-Li,Xue Xing-Ya,&Liang Xin-Miao.(2013).Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,41(2),181-186.
MLA Ding Ling,et al."Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 41.2(2013):181-186.
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