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题名: Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography
作者: Ding Ling1;  Dong Jun1;  Xiao Yuan-Sheng1;  Zhang Xiu-Li1;  Xue Xing-Ya1;  Liang Xin-Miao1
关键词: Protein ;  Reversed phase liquid chromatography ;  Retention equation ;  Predict ;  Optimization
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2013-02-01
DOI: 10.3724/SP.J.1096.2013.20881
卷: 41, 期:2, 页:181-186
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Study on the retention equation for protein in RPLC can contribute to optimizing gradient conditions for protein separation. In this study, five standard proteins, insulin, cytochrome C, lysozyme, transferrin and myoglobin were selected, and their retention time on three reversed-phase columns (Waters Symmetry 300 C-4, Waters Symmetry 300 C-18, C8HC) under six different linear gradients was obtained. According to nonlinear curve fit (CSASS software), the retention equation for five standard proteins on three columns was established. The regression coefficients of the 15 equations were all above 0: 999, indicating that the equation could accurately describe protein retention behavior on RPLC mode. Based on the equation, retention time for 5 standard proteins under other gradient conditions was predicted, and the relative errors for each protein were below 5%. This retention equation could be used in optimizing gradient conditions for protein separation on RPLC, leading to rapid baseline separation of five mixed standard proteins within 20 min.
关键词[WOS]: HYDROPHOBIC INTERACTION CHROMATOGRAPHY ;  AFFINITY-CHROMATOGRAPHY ;  PURIFICATION ;  SEPARATION ;  GRADIENT ;  SYSTEMS ;  ELUTION
语种: 英语
WOS记录号: WOS:000318392300004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137791
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Ding Ling,Dong Jun,Xiao Yuan-Sheng,et al. Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(2):181-186.
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