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题名: 1-(3-Aminopropyl)-3-butylimidazolium bromide for carboxyl group derivatization: potential applications in high sensitivity peptide identification by mass spectrometry
作者: Qiao XiaoQiang1, 2, 3;  Zhou Yuan1;  Hou ChunYan1;  Zhang XiaoDan1;  Yang KaiGuang1;  Zhang LiHua1;  Zhang YuKui1
关键词: 1-(3-aminopropyl)-3-butylimidazolium bromide ;  ionization capacity ;  peptide ;  mass spectrometry analysis ;  derivatization
刊名: SCIENCE CHINA-LIFE SCIENCES
发表日期: 2013-03-01
DOI: 10.1007/s11427-013-4446-8
卷: 56, 期:3, 页:240-245
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
英文摘要: The cationic reagent 1-(3-aminopropyl)-3-butylimidazolium bromide (BAPI) was exploited for the derivatization of carboxyl groups on peptides. Nearly 100% derivatization efficiency was achieved with the synthetic peptide RVYVHPI (RI-7). Furthermore, the peptide derivative was stable in a 0.1% TFA/water solution or a 0.1% (v/v) TFA/acetonitrile/water solution for at least one week. The effect of BAPI derivatization on the ionization of the peptide RI-7 was further investigated, and the detection sensitivity was improved > 42-fold via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), thus outperforming the commercial piperazine derivatization approach. Moreover, the charge states of the peptide were largely increased via BAPI derivatization by electrospray ionization (ESI) MS. The results indicate the potential merits of BAPI derivatization for high sensitivity peptide analysis by MS.
关键词[WOS]: CHEMICAL DERIVATIZATION ;  MS/MS ANALYSIS ;  MALDI-MS ;  IONIZATION ;  PHOSPHOPEPTIDES ;  ENHANCEMENT ;  PROTEINS ;  CAPTURE ;  LIQUID
语种: 英语
WOS记录号: WOS:000316747000007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137821
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Hebei Univ, Key Lab Pharmaceut Qual Control Hebei Prov, Baoding 071002, Peoples R China
3.Hebei Univ, Coll Pharmaceut Sci, Baoding 071002, Peoples R China

Recommended Citation:
Qiao XiaoQiang,Zhou Yuan,Hou ChunYan,et al. 1-(3-Aminopropyl)-3-butylimidazolium bromide for carboxyl group derivatization: potential applications in high sensitivity peptide identification by mass spectrometry[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(3):240-245.
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