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Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke
Liu, Hailin1,2; Sun, Shihao2; Zong, Yongli2; Li, Peng2; Xie, Jianping2
KeywordAntioxidants Cigarette Smoke Reactive Oxygen Species Spectrofluorometer
Source PublicationANALYTICAL LETTERS
2013-02-15
DOI10.1080/00032719.2012.726683
Volume46Issue:4Pages:682-693
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordFREE-RADICALS ; TOBACCO-SMOKE ; N-NITROSAMINES ; BETA-CAROTENE ; IN-VIVO ; MAINSTREAM ; FILTERS ; REDUCE ; 2,2-DIPHENYL-1-PICRYLHYDRAZYL ; RESONANCE
AbstractCigarette smoke can cause cellular oxidative stress that contributes to various adverse health effects associated with smoke exposure, partially due to reactive oxygen species (ROS) present in cigarette smoke. Reduction of abundant ROS in the cigarette mainstream smoke (MSS) is of importance for human health. In this work, a simple, rapid, and reliable fluorescence evaluation of scavenging efficiency of antioxidants as potential filter additives against ROS in cigarette smoke is reported. This method was based on the combination of model glass reactor and a fluorescence assay of ROS in cigarette smoke using dihydrorhodamine 6G (DHR-6G). The antioxidant was added into a glass reactor attached to cigarette filter, which simplified the preparation of combined filter containing additives. The ROS scavenging efficiency of antioxidants was then determined using spectrofluorimetry by the change in fluorescence intensity of whole smoke-bubbled solutions before and after addition of antioxidants into the glass reactor. The proposed method was successfully applied to the determination of ROS scavenging efficiency of several potential additives, such as tert-butylhydroquinone (TBHQ), vitamin C, -carotene, grape seed extract, and Ginkgo biloba extract. Moreover, the relationship between MSS ROS scavenging efficiency and antioxidant activities (DPPH radicals scavenging efficiency and Fe2+ reducing power) of these compounds was also investigated.
Language英语
WOS IDWOS:000315156700011
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137826
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hailin,Sun, Shihao,Zong, Yongli,et al. Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke[J]. ANALYTICAL LETTERS,2013,46(4):682-693.
APA Liu, Hailin,Sun, Shihao,Zong, Yongli,Li, Peng,&Xie, Jianping.(2013).Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke.ANALYTICAL LETTERS,46(4),682-693.
MLA Liu, Hailin,et al."Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke".ANALYTICAL LETTERS 46.4(2013):682-693.
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