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题名: Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke
作者: Liu, Hailin1, 2;  Sun, Shihao2;  Zong, Yongli2;  Li, Peng2;  Xie, Jianping2
关键词: Antioxidants ;  Cigarette smoke ;  Reactive oxygen species ;  Spectrofluorometer
刊名: ANALYTICAL LETTERS
发表日期: 2013-02-15
DOI: 10.1080/00032719.2012.726683
卷: 46, 期:4, 页:682-693
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Cigarette smoke can cause cellular oxidative stress that contributes to various adverse health effects associated with smoke exposure, partially due to reactive oxygen species (ROS) present in cigarette smoke. Reduction of abundant ROS in the cigarette mainstream smoke (MSS) is of importance for human health. In this work, a simple, rapid, and reliable fluorescence evaluation of scavenging efficiency of antioxidants as potential filter additives against ROS in cigarette smoke is reported. This method was based on the combination of model glass reactor and a fluorescence assay of ROS in cigarette smoke using dihydrorhodamine 6G (DHR-6G). The antioxidant was added into a glass reactor attached to cigarette filter, which simplified the preparation of combined filter containing additives. The ROS scavenging efficiency of antioxidants was then determined using spectrofluorimetry by the change in fluorescence intensity of whole smoke-bubbled solutions before and after addition of antioxidants into the glass reactor. The proposed method was successfully applied to the determination of ROS scavenging efficiency of several potential additives, such as tert-butylhydroquinone (TBHQ), vitamin C, -carotene, grape seed extract, and Ginkgo biloba extract. Moreover, the relationship between MSS ROS scavenging efficiency and antioxidant activities (DPPH radicals scavenging efficiency and Fe2+ reducing power) of these compounds was also investigated.
关键词[WOS]: FREE-RADICALS ;  TOBACCO-SMOKE ;  N-NITROSAMINES ;  BETA-CAROTENE ;  IN-VIVO ;  MAINSTREAM ;  FILTERS ;  REDUCE ;  2,2-DIPHENYL-1-PICRYLHYDRAZYL ;  RESONANCE
语种: 英语
WOS记录号: WOS:000315156700011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137826
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China

Recommended Citation:
Liu, Hailin,Sun, Shihao,Zong, Yongli,et al. Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke[J]. ANALYTICAL LETTERS,2013,46(4):682-693.
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