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题名: Stable lsotope-Assisted Lipidomics Combined with Nontargeted lsotopomer Filtering, a Tool to Unravel the Complex Dynamics of Lipid Metabolism
作者: Li, Jia1;  Hoene, Miriam2, 3, 4;  Zhao, Xinjie1;  Chen, Shili1;  Wei, Hai5;  Haering, Hans-Ulrich2, 3, 4;  Lin, Xiaohui5;  Zeng, Zhongda1;  Weigert, Cora2, 3, 4;  Lehmann, Rainer2, 3, 4;  Xu, Guowang1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2013-05-07
DOI: 10.1021/ac400293y
卷: 85, 期:9, 页:4651-4657
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Investigations of complex metabolic mechanisms and networks have become a focus of research in the postgenomic area, thereby creating an increasing demand for sophisticated analytical approaches. One such tool is lipidomics analysis that provides, a detailed picture of the lipid composition of a system at a given time Introducing stable isotopes into the studied system can additionally provide information on the synthesis, transformation and degradation of individual lipid species. However, capturing the entire dynamics of lipid networks is still a challenge. We developed and evaluated a novel strategy for the in-depth analysis of the dynamics of lipid metabolism with the capacity for high molecular specificity and network coverage. The general workflow consists of stable isotope-labeling experiments, ultrahigh-performance liquid chromatography (UHPLC)/high-resolution Orbitrap-mass spectrometry (MS) lipid profiling and data processing by a software tool for global isotopomer filtering and matching. As a proof of concept, this approach was applied to the network-wide mapping of dynamic lipid metabolism in primary human skeletal muscle cells cultured for 4, 12, and 24 h with [U-C-13]-palmitate. In the myocellular lipid extracts, 692 isotopomers were detected that could be assigned to 203 labeled lipid species spanning 12 lipid (sub)classes. Interestingly, some lipid classes showed high turnover rates but stable total amounts while the amount of others increased in the course of palmitate treatment. The novel strategy presented here has the potential to open new detailed insights into the dynamics of lipid metabolism that may lead to a better understanding of physiological mechanisms and metabolic perturbations.
关键词[WOS]: CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY ;  IN-VIVO ;  INSULIN-RESISTANCE ;  HUMAN MYOTUBES ;  FATTY-ACIDS ;  MUSCLE ;  METABOLOMICS ;  CERAMIDES ;  DATABASE ;  LIPOTOXICITY
语种: 英语
WOS记录号: WOS:000318756100063
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137832
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Univ Tubingen Hosp, Dept Internal Med 4, Div Clin Chem & Pathobiochem, Tubingen, Germany
3.Univ Tubingen, Paul Langerhans Inst Tuebingen, Helmholtz Ctr Munich, Inst Diabet Res & Metab Dis, Tubingen, Germany
4.German Ctr Diabet Res DZD, Tubingen, Germany
5.Dalian Univ Technol, Sch Comp Sci & Technol, Dalian, Peoples R China

Recommended Citation:
Li, Jia,Hoene, Miriam,Zhao, Xinjie,et al. Stable lsotope-Assisted Lipidomics Combined with Nontargeted lsotopomer Filtering, a Tool to Unravel the Complex Dynamics of Lipid Metabolism[J]. ANALYTICAL CHEMISTRY,2013,85(9):4651-4657.
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