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题名: Baohuoside I production through enzyme hydrolysis and parameter optimization by using response surface and subset selection
作者: Yang, QianXu1;  Wang, Li1;  Zhang, LiangXiao1;  Xiao, HongBin1
关键词: Dextranase ;  Baohuoside I ;  Subset selection ;  Enzyme hydrolysis ;  Optimization
刊名: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
发表日期: 2013-06-01
DOI: 10.1016/j.molcatb.2013.01.017
卷: 90, 页:132-138
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ;  Chemistry, Physical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A rapid and efficient baohuoside I preparation method was established. A uniform design coupled with subset selection was employed to determine pH, reaction time, and temperature parameters, as well as dextranase hydrolysis efficiency. Hydrolysis parameters were optimized using response surface and subset selection. Our results showed that pH plays an important role in the hydrolysis reaction within a relatively narrow range (pH 4-7). Temperature was the secondary factor, which was positively correlated with conversion rate. A 3-h reaction time was sufficient. Finally, a relatively good hydrolysis parameters were found, and their effectiveness was verified. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
关键词[WOS]: HERBA-EPIMEDII EXTRACT ;  ICARISIDE-II ;  VARIABLE-SELECTION ;  CROSS-VALIDATION ;  CANCER CELLS ;  ICARIIN ;  EXCRETION ;  MODEL ;  VITRO ;  MICE
语种: 英语
WOS记录号: WOS:000316924800021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137834
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Yang, QianXu,Wang, Li,Zhang, LiangXiao,et al. Baohuoside I production through enzyme hydrolysis and parameter optimization by using response surface and subset selection[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2013,90:132-138.
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