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题名: Development of a rapid method for the simultaneous separation and determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its N- and O-glucuronides in human urine by liquid chromatography-tandem mass spectrometry
作者: Yao, Li1;  Zheng, Saijing2;  Guan, Yafeng1, 3;  Yang, Jun1;  Liu, Baizhan2;  Wang, Weimiao2;  Zhu, Xiaolan1
关键词: 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol ;  Metabolism ;  Pentafluorophenyl column ;  Liquid chromatography-tandem mass spectrometry ;  Solid-phase extraction ;  Human urine
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2013-07-25
DOI: 10.1016/j.aca.2013.06.034
卷: 788, 页:61-67
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Determination of the tobacco-specific nitrosamine metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanol (NNAL) and its N- and O-glucuronides (NNAL-N-Gluc and NNAL-O-Gluc) is important for toxicology analysis of tobacco smoke induced carcinogenicity and the understanding of detoxification mechanisms of the carcinogenic nitrosamine in humans. But previously reported indirect measurement methods involving enzymolysis and base treatment steps were tedious and time-consuming. In this work, a direct measurement method for simultaneous determination of urinary NNAL, NNAL-N-Gluc and NNAL-O-Gluc by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a single run was developed for the first time without the need to perform enzymatic or base hydrolysis. Urine samples were purified using dichloromethane and further extracted by solid-phase extraction. Then they were analyzed by LC-MS/MS operated in electrospray positive ionization mode. Chromatographic separation was achieved on a Phenomenex Kinetex PFP column within 6 min. The proposed method was validated and the results demonstrated that the method can produce satisfactory recoveries and reproducibility for the analytes. The applicability of this newly developed method was investigated for the simultaneous analysis of the three metabolites in smokers' urine and the obtained results were comparable to those detected using the conventional enzymolysis method. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: TOBACCO-SPECIFIC NITROSAMINE ;  PENTAFLUOROPHENYLPROPYL STATIONARY-PHASE ;  MOLECULARLY IMPRINTED POLYMER ;  HUMAN PLASMA ;  INTERNAL STANDARDS ;  NATURAL-PRODUCTS ;  PERFORMANCE ;  VALIDATION ;  EXTRACTION ;  ISOMERS
语种: 英语
WOS记录号: WOS:000321497700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137888
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
2.China Natl Tobacco Corp, Key Lab Cigarette Smoke Res, Shanghai 200082, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Microinstrumentat & Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Yao, Li,Zheng, Saijing,Guan, Yafeng,et al. Development of a rapid method for the simultaneous separation and determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its N- and O-glucuronides in human urine by liquid chromatography-tandem mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2013,788:61-67.
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