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Investigation of the Relationship between the Metabolic Profile of Tobacco Leaves in Different Planting Regions and Climate Factors Using a Pseudotargeted Method Based on Gas Chromatography/Mass Spectrometry
Zhao, Yanni1; Zhao, Chunxia1; Lu, Xin1; Zhou, Huina2; Li, Yanli1; Zhou, Jia1; Chang, Yuwei1; Zhang, Junjie1; Jin, Lifeng2; Lin, Fucheng2; Xu, Guowang1
KeywordMetabolic Profiling Pseudotargeted Climate Tobacco Leaf Gc/ms Metabolomics
Source PublicationJOURNAL OF PROTEOME RESEARCH
2013-11-01
DOI10.1021/pr400799a
Volume12Issue:11Pages:5072-5083
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordTRANSGENIC RICE ; GC-MS ; TOLERANCE ; STRESS ; RESPONSES ; LEAF ; GENE ; L. ; OVEREXPRESSION ; DEHYDRATION
AbstractAn improved pseudotargeted method using gas chromatography/mass spectrometry (GC/MS) was developed to investigate the metabolic profile of tobacco leaves from three planting regions (Yunnan, Guizhou, and Henan provinces). The analytical characteristics of the method with regard to reproducibility, precision, linearity, and stability were satisfactory for metabolic profiling study. Partial least-squares-discriminant analysis and hierarchical cluster analysis demonstrated that the metabolic profiles of tobacco from the Yunnan and Guizhou regions were different from that from the Henan province. The amino acid (e.g., phenylalanine, leucine, and tyrosine) and carbohydrate (e.g., fructose, trehalose, and sucrose) contents were the highest in Henan tobacco. The highest contents of organic acids (e.g., isocitrate, citrate, and fumarate) of the TCA cycle and antioxidants (e.g., quinate, chlorogenic acid, and ascorbate) were found in Guizhou tobacco. The correlation coefficients between metabolite content and climate factors (rainfall, sunshine, and temperature) demonstrated that drought facilitated the accumulation of sugars and amino acids. The content of TCA cycle intermediates could be influenced by multiple climate factors. This study demonstrates that the pseudotargeted method with GC/MS is suitable for the investigation of the metabolic profiling of tobacco leaves and the assessment of differential metabolite levels related to the growing regions.
Language英语
WOS IDWOS:000326615000031
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/137908
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou 450001, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Yanni,Zhao, Chunxia,Lu, Xin,et al. Investigation of the Relationship between the Metabolic Profile of Tobacco Leaves in Different Planting Regions and Climate Factors Using a Pseudotargeted Method Based on Gas Chromatography/Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(11):5072-5083.
APA Zhao, Yanni.,Zhao, Chunxia.,Lu, Xin.,Zhou, Huina.,Li, Yanli.,...&Xu, Guowang.(2013).Investigation of the Relationship between the Metabolic Profile of Tobacco Leaves in Different Planting Regions and Climate Factors Using a Pseudotargeted Method Based on Gas Chromatography/Mass Spectrometry.JOURNAL OF PROTEOME RESEARCH,12(11),5072-5083.
MLA Zhao, Yanni,et al."Investigation of the Relationship between the Metabolic Profile of Tobacco Leaves in Different Planting Regions and Climate Factors Using a Pseudotargeted Method Based on Gas Chromatography/Mass Spectrometry".JOURNAL OF PROTEOME RESEARCH 12.11(2013):5072-5083.
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