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题名: Investigation of the Relationship between the Metabolic Profile of Tobacco Leaves in Different Planting Regions and Climate Factors Using a Pseudotargeted Method Based on Gas Chromatography/Mass Spectrometry
作者: Zhao, Yanni1;  Zhao, Chunxia1;  Lu, Xin1;  Zhou, Huina2;  Li, Yanli1;  Zhou, Jia1;  Chang, Yuwei1;  Zhang, Junjie1;  Jin, Lifeng2;  Lin, Fucheng2;  Xu, Guowang1
关键词: metabolic profiling ;  pseudotargeted ;  climate ;  tobacco leaf ;  GC/MS ;  metabolomics
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2013-11-01
DOI: 10.1021/pr400799a
卷: 12, 期:11, 页:5072-5083
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: An improved pseudotargeted method using gas chromatography/mass spectrometry (GC/MS) was developed to investigate the metabolic profile of tobacco leaves from three planting regions (Yunnan, Guizhou, and Henan provinces). The analytical characteristics of the method with regard to reproducibility, precision, linearity, and stability were satisfactory for metabolic profiling study. Partial least-squares-discriminant analysis and hierarchical cluster analysis demonstrated that the metabolic profiles of tobacco from the Yunnan and Guizhou regions were different from that from the Henan province. The amino acid (e.g., phenylalanine, leucine, and tyrosine) and carbohydrate (e.g., fructose, trehalose, and sucrose) contents were the highest in Henan tobacco. The highest contents of organic acids (e.g., isocitrate, citrate, and fumarate) of the TCA cycle and antioxidants (e.g., quinate, chlorogenic acid, and ascorbate) were found in Guizhou tobacco. The correlation coefficients between metabolite content and climate factors (rainfall, sunshine, and temperature) demonstrated that drought facilitated the accumulation of sugars and amino acids. The content of TCA cycle intermediates could be influenced by multiple climate factors. This study demonstrates that the pseudotargeted method with GC/MS is suitable for the investigation of the metabolic profiling of tobacco leaves and the assessment of differential metabolite levels related to the growing regions.
关键词[WOS]: TRANSGENIC RICE ;  GC-MS ;  TOLERANCE ;  STRESS ;  RESPONSES ;  LEAF ;  GENE ;  L. ;  OVEREXPRESSION ;  DEHYDRATION
语种: 英语
WOS记录号: WOS:000326615000031
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137908
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou 450001, Peoples R China

Recommended Citation:
Zhao, Yanni,Zhao, Chunxia,Lu, Xin,et al. Investigation of the Relationship between the Metabolic Profile of Tobacco Leaves in Different Planting Regions and Climate Factors Using a Pseudotargeted Method Based on Gas Chromatography/Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(11):5072-5083.
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