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题名: Characterization of a new alginate lyase from newly isolated Flavobacterium sp S20
作者: Huang, Lishuxin1, 2;  Zhou, Jungang3;  Li, Xiao4;  Peng, Qiang1, 2;  Lu, Hong3;  Du, Yuguang1
关键词: Flavobacterium ;  Alginate lyase ;  Saccharification ;  Large-scale preparation ;  Alginate oligosaccharides
刊名: JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2013
DOI: 10.1007/s10295-012-1210-1
卷: 40, 期:1, 页:113-122
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Alginate lyase is a promising biocatalyst because of its application in saccharification of alginate for the production of biochemicals and renewable biofuels. This study described the isolation of a new alginate metabolizing bacterium, Flavobacterium sp. S20, from sludge samples and the characterization of its alginate lyase Alg2A. The alginate lyase gene, alg2A, was obtained by constructing and screening the genomic library of the strain S20 and overexpressed in Escherichia coli. Substrate specificity assays indicated Alg2A preferred poly-alpha-l-guluronate as a substrate over poly-beta-d-mannuronate. In the saccharification process of a high content (10 %, w/v) of sodium alginate, the recombinant alginate lyase Alg2A yielded 152 of mM the reducing sugars after 69 h of reaction, and the amounts of oligosaccharides with a different degree of polymerization (DP) generated by Alg2A gradually accumulated without significant variation in the distribution of oligosaccharide compositions. These results indicated that Alg2A possessed high enzymatic capability for saccharifying the alginate, which could be used in saccharifying the alginate biomass prior to the main fermentation process for biofuels. In addition, Alg2A had a different endolytic reaction mode from both the two commercial alginate lyases and other alginate lyases from polysaccharide lyase family 7 owing to high yields of penta-, hex-, and hepta-saccharides in the hydrolysis products of Alg2A. Thus, Alg2A could be a good tool for the large-scale preparation of alginate oligosaccharides with high DP.
关键词[WOS]: SACCHAROPHAGUS DEGRADANS ;  PSEUDOMONAS-AERUGINOSA ;  OLIGOALGINATE LYASE ;  ESCHERICHIA-COLI ;  GULURONATE LYASE ;  PURIFICATION ;  ACID ;  OLIGOSACCHARIDES ;  OVEREXPRESSION ;  SUGAR
语种: 英语
WOS记录号: WOS:000313019300012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137931
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Res Grp, Liaoning Prov Key Lab Carbohydrates,CAS, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Fudan Univ, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
4.NW Univ Xian, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Shan Xi Prov Key Lab Biotechnol,Life Sci Coll, Xian 710069, Peoples R China

Recommended Citation:
Huang, Lishuxin,Zhou, Jungang,Li, Xiao,et al. Characterization of a new alginate lyase from newly isolated Flavobacterium sp S20[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2013,40(1):113-122.
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