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题名: Application of a strong anion exchange material in electrostatic repulsion-hydrophilic interaction chromatography for selective enrichment of glycopeptides
作者: Cao, Liwei1;  Yu, Long1;  Guo, Zhimou1;  Li, Xiuling1;  Xue, Xinya1;  Liang, Xinmiao1
关键词: SAX ;  ERLIC ;  Glycopeptides ;  Enrichment ;  Mass spectrometry
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2013-07-19
DOI: 10.1016/j.chroma.2013.05.037
卷: 1299, 页:18-24
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Glycoproteins are involved in various cellular activities, including inter- and extracellular signaling. However, glycopeptide signals are significantly suppressed by coeluting non-glycosylated peptides in mass spectrometry-based analysis. For detailed elucidation of the biological functions of glycoproteins, selective enrichment of glycopeptides from non-glycosylated peptides is crucial. In the present study, a SAX material, XCharge SAX, was used in a column in the ERLIC mode with the aim of specifically enriching glycopeptides. Enrichment conditions were initially optimized, and selectivity, glycosylation heterogeneity coverage and detection sensitivity of XCharge SAX were subsequently assessed. In the selectivity assessment, glycopeptides were effectively isolated from a peptide mixture (human serum immunoglobulin G (IgG) and human serum albumin digests) and a tryptic digest of human serum using XCharge SAX. In the evaluation of glycosylation heterogeneity coverage, five glycosites and eleven glyco peptides from horseradish peroxidase were identified after enrichment with XCharge SAX. In detection sensitivity assessment, glycopeptides within four orders of magnitude were identified after enrichment with XCharge SAX. In addition, volatile solvents were used in the loading and eluting buffers so that desalting was not necessary for ERLIC fractions. Our results collectively support the utility of XCharge SAX as a suitable chromatographic material for global glycosylation site analysis. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: N-LINKED GLYCOPROTEINS ;  TANDEM MASS-SPECTROMETRY ;  SOLID-PHASE EXTRACTION ;  GLYCOSYLATION HETEROGENEITY ;  BIOMARKER DISCOVERY ;  AFFINITY CAPTURE ;  FACILE SYNTHESIS ;  IDENTIFICATION ;  SEPARATION ;  DATABASE
语种: 英语
WOS记录号: WOS:000321424000003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/137932
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Cao, Liwei,Yu, Long,Guo, Zhimou,et al. Application of a strong anion exchange material in electrostatic repulsion-hydrophilic interaction chromatography for selective enrichment of glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1299:18-24.
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