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Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes
Chang, Yuwei1; Zhao, Chunxia1; Zhu, Zhen2; Wu, Zeming1; Zhou, Jia1; Zhao, Yanni1; Lu, Xin1; Xu, Guowang1
KeywordLiquid Chromatography Mass Spectrometry Metabolic Profiling Transgenic Rice Unintended Effects Metabolomics
Source PublicationPLANT MOLECULAR BIOLOGY
2012-03-01
DOI10.1007/s11103-012-9876-3
Volume78Issue:4-5Pages:477-487
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS KeywordFLIGHT MASS-SPECTROMETRY ; MAIZE GRAIN ; NONTRANSGENIC COUNTERPART ; BACILLUS-THURINGIENSIS ; SAFETY EVALUATION ; ORYZA-SATIVA ; PLANTS ; PERFORMANCE ; ENVIRONMENT ; EXPRESSION
AbstractAs a primary characteristic of substantial equivalence, the evaluation of unintended effects of genetically modified plants has been evolving into an important field of research. In this study, a metabolic profiling method for rice seeds was developed using rapid resolution liquid chromatography/quadrupole time-of-flight mass spectrometry. The analytical properties of the method, including the linearity, reproducibility, intra-day precision and inter-day precision, were investigated and were found to be satisfactory. The method was then applied to investigate the differences between transgenic rice and its native counterparts, in addition to the differences found between native rice with different sowing dates or locations. Global metabolic phenotype differences were visualized, and metabolites from different discriminated groups were discovered using multivariate data analysis. The results indicated that environmental factors played a greater role than gene modification for most metabolites, including tryptophan, 9,10,13-trihydroxyoctadec-11-enoic acid, and lysophosphatidylethanolamine 16:0. The concentrations of phytosphingosine, palmitic acid, 5-hydroxy-2-octadenoic acid and three other unidentified metabolites varied slightly due to gene modification.
Language英语
WOS IDWOS:000302252300012
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138029
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Chang, Yuwei,Zhao, Chunxia,Zhu, Zhen,et al. Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes[J]. PLANT MOLECULAR BIOLOGY,2012,78(4-5):477-487.
APA Chang, Yuwei.,Zhao, Chunxia.,Zhu, Zhen.,Wu, Zeming.,Zhou, Jia.,...&Xu, Guowang.(2012).Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes.PLANT MOLECULAR BIOLOGY,78(4-5),477-487.
MLA Chang, Yuwei,et al."Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes".PLANT MOLECULAR BIOLOGY 78.4-5(2012):477-487.
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