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题名: The Conformational Polymorphism of the Green Fluorescent Protein
作者: Tan, Haidong1;  Li, Yueguang2;  Chen, Ling3;  Kudoh, Takayuki3;  Kasai, Tomonari3;  Seno, Masaharu3
关键词: green fluorescent protein ;  conformational polymorphism ;  glutathione-S-transferase ;  native polyacrylamide gel electrophoresis ;  MALDI-TOF MS ;  circular dichroism spectra
刊名: MOLECULAR BIOLOGY
发表日期: 2012-02-01
DOI: 10.1134/S0026893311060045
卷: 46, 期:1, 页:142-148
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Green fluorescent protein (GFPuv) has been widely used as a reporter fused to individual targeting sequences. However, its state in liquid and its effect on other proteins are still unclear. The conformational polymorphisms of glutathione-S-transferase-green fluorescent protein (GST-GFPuv), GFPuv and GST were analyzed by native polyacrylamide gel, indicating that GST was in many different states while GFPuv and GST-GFPuv were only in four and two slightly different states. Four different circular dichroism spectra were obtained from the GFPuv polymorphisms. The single molecular behavior of GST-GFPuv and GFPuv was also characterized by MALDI-TOF MS. Thus, we demonstrated that: (1) there might be four different structural polymorphisms for the native GFPuv; (2) GFPuv could reduce its partner's polymorphism as a fusion protein. Although GFPuv had many merits as a reporter, its unreliability was found in the study. DOI: 10.1134/S0026893311060045
关键词[WOS]: LIVING CELLS ;  THERMAL-STABILITY ;  DYNAMICS ;  GFP ;  SPECTROSCOPY ;  EXPRESSION ;  MOLECULES ;  TRACKING ;  REPORTER ;  FUSION
语种: 英语
WOS记录号: WOS:000302960100017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138040
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.CAS, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Tianjin 4th Ctr Hosp, Dept Gen Surg, Tianjin 300140, Peoples R China
3.Okayama Univ, Grad Sch Nat Sci & Technol, Dept Med & Bioengn Sci, Okayama 7008530, Japan

Recommended Citation:
Tan, Haidong,Li, Yueguang,Chen, Ling,et al. The Conformational Polymorphism of the Green Fluorescent Protein[J]. MOLECULAR BIOLOGY,2012,46(1):142-148.
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