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Hollow fiber-based liquid-liquid-liquid micro-extraction with osmosis: II. Application to quantification of endogenous gibberellins in rice plant
Wu, Qian; Wu, Dapeng; Duan, Chunfeng; Shen, Zheng; Guan, Yafeng
KeywordHollow Fiber-based Liquid-liquid-liquid Micro-extraction (Hf-lllme) With Osmosis High Performance Liquid Chromatography (Hplc)-triple Quadrupole Mass Spectrometry (Ms/ms) Endogenous Gibberellins Rice Plant
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2012-11-23
DOI10.1016/j.chroma.2012.09.066
Volume1265Pages:17-23
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; CAPILLARY-ELECTROPHORESIS ; QUANTITATIVE-ANALYSIS ; SINGLE DROP ; MICROEXTRACTION ; CHROMATOGRAPHY ; ACID ; FRUITS ; WATER
AbstractThe phenomenon and benefits of osmosis in hollow fiber-based liquid-liquid-liquid micro-extraction (HF-LLLME) were theoretically discussed in part I of this study. In this work, HF-LLLME with osmosis was coupled with high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-triple quadrupole MS/MS) to analyze eight gibberellins (gibberellin A(1), gibberellin A(3), gibberellin A(4), gibberellin A(7), gibberellin A(8), gibberellin A(9), gibberellin A(19) and gibberellin A(20)) in rice plant samples. According to the theory of HF-LLLME with osmosis, single factor experiments, orthogonal design experiments and mass transfer simulation of extraction process were carried out to select the optimal conditions. Cyclohexanol - n-octanol (1:3, v/v) was selected as organic membrane. Donor phase of 12 mL was adjusted to pH 2 and 20% NaCl (w/v) was added. Acceptor phase with an initial volume of 20 mu L. was the solution of 0.12 mol L-1 Na2CO3-NaHCO3 buffer (pH 9). Temperature was chosen to be 30 degrees C and extraction time was selected to be 90 min. Under optimized conditions, this method provided good linearity (r, 0.99552-0.99991) and low limits of detection (0.0016-0.061 ng mL(-1)). Finally, this method was applied to the analysis of endogenous gibberellins from plant extract which was obtained with traditional solvent extraction of rice plant tissues, and the relative recoveries were from 62% to 166%. (C) 2012 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000311011000003
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138045
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wu, Qian,Wu, Dapeng,Duan, Chunfeng,et al. Hollow fiber-based liquid-liquid-liquid micro-extraction with osmosis: II. Application to quantification of endogenous gibberellins in rice plant[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1265:17-23.
APA Wu, Qian,Wu, Dapeng,Duan, Chunfeng,Shen, Zheng,&Guan, Yafeng.(2012).Hollow fiber-based liquid-liquid-liquid micro-extraction with osmosis: II. Application to quantification of endogenous gibberellins in rice plant.JOURNAL OF CHROMATOGRAPHY A,1265,17-23.
MLA Wu, Qian,et al."Hollow fiber-based liquid-liquid-liquid micro-extraction with osmosis: II. Application to quantification of endogenous gibberellins in rice plant".JOURNAL OF CHROMATOGRAPHY A 1265(2012):17-23.
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