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题名: Hollow fiber-based liquid-liquid-liquid micro-extraction with osmosis: II. Application to quantification of endogenous gibberellins in rice plant
作者: Wu, Qian1;  Wu, Dapeng1;  Duan, Chunfeng1;  Shen, Zheng1;  Guan, Yafeng1
关键词: Hollow fiber-based liquid-liquid-liquid micro-extraction (HF-LLLME) with osmosis ;  High performance liquid chromatography (HPLC)-triple quadrupole mass spectrometry (MS/MS) ;  Endogenous gibberellins ;  Rice plant
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2012-11-23
DOI: 10.1016/j.chroma.2012.09.066
卷: 1265, 页:17-23
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The phenomenon and benefits of osmosis in hollow fiber-based liquid-liquid-liquid micro-extraction (HF-LLLME) were theoretically discussed in part I of this study. In this work, HF-LLLME with osmosis was coupled with high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-triple quadrupole MS/MS) to analyze eight gibberellins (gibberellin A(1), gibberellin A(3), gibberellin A(4), gibberellin A(7), gibberellin A(8), gibberellin A(9), gibberellin A(19) and gibberellin A(20)) in rice plant samples. According to the theory of HF-LLLME with osmosis, single factor experiments, orthogonal design experiments and mass transfer simulation of extraction process were carried out to select the optimal conditions. Cyclohexanol - n-octanol (1:3, v/v) was selected as organic membrane. Donor phase of 12 mL was adjusted to pH 2 and 20% NaCl (w/v) was added. Acceptor phase with an initial volume of 20 mu L. was the solution of 0.12 mol L-1 Na2CO3-NaHCO3 buffer (pH 9). Temperature was chosen to be 30 degrees C and extraction time was selected to be 90 min. Under optimized conditions, this method provided good linearity (r, 0.99552-0.99991) and low limits of detection (0.0016-0.061 ng mL(-1)). Finally, this method was applied to the analysis of endogenous gibberellins from plant extract which was obtained with traditional solvent extraction of rice plant tissues, and the relative recoveries were from 62% to 166%. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  SOLID-PHASE EXTRACTION ;  CAPILLARY-ELECTROPHORESIS ;  QUANTITATIVE-ANALYSIS ;  SINGLE DROP ;  MICROEXTRACTION ;  CHROMATOGRAPHY ;  ACID ;  FRUITS ;  WATER
语种: 英语
WOS记录号: WOS:000311011000003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138045
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Wu, Qian,Wu, Dapeng,Duan, Chunfeng,et al. Hollow fiber-based liquid-liquid-liquid micro-extraction with osmosis: II. Application to quantification of endogenous gibberellins in rice plant[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1265:17-23.
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