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Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment
Qu, Yanyan1,2; Liu, Jianxi1,2; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
关键词Core-shell Particles Glycopeptides Nanoparticles Polymers
刊名CHEMISTRY-A EUROPEAN JOURNAL
2012-07-01
DOI10.1002/chem.201103514
18期:29页:9056-9062
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]N-LINKED GLYCOPROTEINS ; MASS-SPECTROMETRY ; GLYCOSYLATION SITES ; MICROSPHERES ; MONODISPERSE ; CANCER ; IDENTIFICATION ; SEPARATION ; PROTEINS
英文摘要The boronic acid-functionalized coreshell polymer nanoparticles, poly(N,N-methylenebisacrylamide-co-methacrylic acid)@4-vinylphenylboronic acid (poly(MBA-co-MAA)@VPBA), were successfully synthesized for enriching glycosylated peptides. Such nanoparticles were composed of a hydrophilic polymer core prepared by distillation precipitation polymerization (DPP) and a boronic acid-functionalized shell designed for capturing glycopeptides. Owing to the relatively large amount of residual vinyl groups introduced by DPP on the core surface, the VPBA monomer was coated with high efficiency, working as the shell. Moreover, the overall polymerization route, especially the use of DPP, made the synthesis of nanoparticles facile and time-saving. With the poly(MBA-co-MAA)@VPBA nanoparticles, 18 glycopeptides from horseradish peroxidase (HRP) digest were captured and identified by MALDI-TOF mass spectrometric analysis, relative to eight glycopeptides enriched by using commercially available meta-aminophenylboronic acid agarose under the same conditions. When the concentration of the HRP digest was decreased to as low as 5 nmol, glycopeptides could still be selectively isolated by the prepared nanoparticles. Our results demonstrated that the synthetic poly(MBA-co-MAA)@VPBA nanoparticles might be a promising selective enrichment material for glycoproteome analysis.
语种英语
WOS记录号WOS:000306221900027
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被引频次:71[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138046
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Qu, Yanyan,Liu, Jianxi,Yang, Kaiguang,et al. Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment[J]. CHEMISTRY-A EUROPEAN JOURNAL,2012,18(29):9056-9062.
APA Qu, Yanyan,Liu, Jianxi,Yang, Kaiguang,Liang, Zhen,Zhang, Lihua,&Zhang, Yukui.(2012).Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment.CHEMISTRY-A EUROPEAN JOURNAL,18(29),9056-9062.
MLA Qu, Yanyan,et al."Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment".CHEMISTRY-A EUROPEAN JOURNAL 18.29(2012):9056-9062.
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