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题名: Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment
作者: Qu, Yanyan1, 2;  Liu, Jianxi1, 2;  Yang, Kaiguang1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
关键词: core-shell particles ;  glycopeptides ;  nanoparticles ;  polymers
刊名: CHEMISTRY-A EUROPEAN JOURNAL
发表日期: 2012-07-01
DOI: 10.1002/chem.201103514
卷: 18, 期:29, 页:9056-9062
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: The boronic acid-functionalized coreshell polymer nanoparticles, poly(N,N-methylenebisacrylamide-co-methacrylic acid)@4-vinylphenylboronic acid (poly(MBA-co-MAA)@VPBA), were successfully synthesized for enriching glycosylated peptides. Such nanoparticles were composed of a hydrophilic polymer core prepared by distillation precipitation polymerization (DPP) and a boronic acid-functionalized shell designed for capturing glycopeptides. Owing to the relatively large amount of residual vinyl groups introduced by DPP on the core surface, the VPBA monomer was coated with high efficiency, working as the shell. Moreover, the overall polymerization route, especially the use of DPP, made the synthesis of nanoparticles facile and time-saving. With the poly(MBA-co-MAA)@VPBA nanoparticles, 18 glycopeptides from horseradish peroxidase (HRP) digest were captured and identified by MALDI-TOF mass spectrometric analysis, relative to eight glycopeptides enriched by using commercially available meta-aminophenylboronic acid agarose under the same conditions. When the concentration of the HRP digest was decreased to as low as 5 nmol, glycopeptides could still be selectively isolated by the prepared nanoparticles. Our results demonstrated that the synthetic poly(MBA-co-MAA)@VPBA nanoparticles might be a promising selective enrichment material for glycoproteome analysis.
关键词[WOS]: N-LINKED GLYCOPROTEINS ;  MASS-SPECTROMETRY ;  GLYCOSYLATION SITES ;  MICROSPHERES ;  MONODISPERSE ;  CANCER ;  IDENTIFICATION ;  SEPARATION ;  PROTEINS
语种: 英语
WOS记录号: WOS:000306221900027
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138046
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Qu, Yanyan,Liu, Jianxi,Yang, Kaiguang,et al. Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment[J]. CHEMISTRY-A EUROPEAN JOURNAL,2012,18(29):9056-9062.
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