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Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment
Qu, Yanyan1,2; Liu, Jianxi1,2; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
KeywordCore-shell Particles Glycopeptides Nanoparticles Polymers
Source PublicationCHEMISTRY-A EUROPEAN JOURNAL
2012-07-01
DOI10.1002/chem.201103514
Volume18Issue:29Pages:9056-9062
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordN-LINKED GLYCOPROTEINS ; MASS-SPECTROMETRY ; GLYCOSYLATION SITES ; MICROSPHERES ; MONODISPERSE ; CANCER ; IDENTIFICATION ; SEPARATION ; PROTEINS
AbstractThe boronic acid-functionalized coreshell polymer nanoparticles, poly(N,N-methylenebisacrylamide-co-methacrylic acid)@4-vinylphenylboronic acid (poly(MBA-co-MAA)@VPBA), were successfully synthesized for enriching glycosylated peptides. Such nanoparticles were composed of a hydrophilic polymer core prepared by distillation precipitation polymerization (DPP) and a boronic acid-functionalized shell designed for capturing glycopeptides. Owing to the relatively large amount of residual vinyl groups introduced by DPP on the core surface, the VPBA monomer was coated with high efficiency, working as the shell. Moreover, the overall polymerization route, especially the use of DPP, made the synthesis of nanoparticles facile and time-saving. With the poly(MBA-co-MAA)@VPBA nanoparticles, 18 glycopeptides from horseradish peroxidase (HRP) digest were captured and identified by MALDI-TOF mass spectrometric analysis, relative to eight glycopeptides enriched by using commercially available meta-aminophenylboronic acid agarose under the same conditions. When the concentration of the HRP digest was decreased to as low as 5 nmol, glycopeptides could still be selectively isolated by the prepared nanoparticles. Our results demonstrated that the synthetic poly(MBA-co-MAA)@VPBA nanoparticles might be a promising selective enrichment material for glycoproteome analysis.
Language英语
WOS IDWOS:000306221900027
Citation statistics
Cited Times:78[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138046
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Qu, Yanyan,Liu, Jianxi,Yang, Kaiguang,et al. Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment[J]. CHEMISTRY-A EUROPEAN JOURNAL,2012,18(29):9056-9062.
APA Qu, Yanyan,Liu, Jianxi,Yang, Kaiguang,Liang, Zhen,Zhang, Lihua,&Zhang, Yukui.(2012).Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment.CHEMISTRY-A EUROPEAN JOURNAL,18(29),9056-9062.
MLA Qu, Yanyan,et al."Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment".CHEMISTRY-A EUROPEAN JOURNAL 18.29(2012):9056-9062.
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