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题名: Facile Preparation of Zwitterionic Organic-Silica Hybrid Monolithic Capillary Column with an Improved "One-Pot" Approach for Hydrophilic-Interaction Liquid Chromatography (HILIC)
作者: Lin, Hui1, 2;  Ou, Junjie1;  Zhang, Zhenbin1, 2;  Dong, Jing1;  Wu, Minghuo1, 3;  Zou, Hanfa1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2012-03-20
DOI: 10.1021/ac3001429
卷: 84, 期:6, 页:2721-2728
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A simple single-step thermal-treatment "one-pot" approach for the preparation of organic-silica hybrid capillary monolithic columns is described. In this improved method, the cross-linker vinyltrimethoxysilane (VTMS) was replaced by 3-methacryloxypropyltrimethoxysilane (gamma-MAPS), which is more active in polymerization reactions, and only one thermal treatment step was required in the preparation of hybrid monoliths. Two zwitterionic organic-silica monolithic columns were successfully synthesized by using [2-(methacryloyloxy)-ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (MSA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) as the organic monomers. The effects of the tetramethoxysilane (TMOS)/gamma-MAPS molar ratio, content of monomer, composition of porogenic solvent, and reaction temperature on the morphologies of the hybrid monoliths were investigated. The MSA-silica and MPC-silica hybrid monolithic columns exhibited good permeability and good mechanical stability. The monolithic columns were used for the separation of polar compounds by capillary hydrophilic-interaction chromatography (cHILIC). A typical HILIC retention mechanism was observed at higher organic solvent contents (>50% ACN). The MSA monoliths were further investigated in the separation of various neutral, basic, and acidic analytes, as well as small peptides, by capillary liquid chromatography (cLC), and high efficiency and satisfactory reproducibility were achieved. In addition, the analysis of a tryptic digest of bovine serum albumin (BSA) by cLC tandem mass spectrometry (cLC-MS/MS) with an MSA monolith further demonstrated its potential in the separation of biological samples.
关键词[WOS]: POLAR STATIONARY PHASES ;  MASS-SPECTROMETRY ;  MIXED-MODE ;  GLYCOPEPTIDE ENRICHMENT ;  PEPTIDE SEPARATION ;  PROTEOME ANALYSIS ;  N-GLYCOPEPTIDES ;  NANO-LC ;  ELECTROCHROMATOGRAPHY ;  PERFORMANCE
语种: 英语
WOS记录号: WOS:000301634500016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138054
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Nanchang Hangkong Univ, Coll Environm & Chem Engn, Nanchang 330063, Peoples R China

Recommended Citation:
Lin, Hui,Ou, Junjie,Zhang, Zhenbin,et al. Facile Preparation of Zwitterionic Organic-Silica Hybrid Monolithic Capillary Column with an Improved "One-Pot" Approach for Hydrophilic-Interaction Liquid Chromatography (HILIC)[J]. ANALYTICAL CHEMISTRY,2012,84(6):2721-2728.
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