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Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor beta 1 (TGF-beta 1) in Epithelial Cell Lines
Xu, Qingsong1,2; Isaji, Tomoya1; Lu, Yingying1; Gu, Wei1; Kondo, Madoka1; Fukuda, Tomohiko1; Du, Yuguang2; Gu, Jianguo1
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2012-05-11
DOI10.1074/jbc.M111.262154
287期:20页:16563-16574
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]E-CADHERIN ; GLYCOSYLATION AFFECTS ; TUMOR PROGRESSION ; BREAST-CARCINOMA ; PROPELLER DOMAIN ; BISECTING GLCNAC ; MOUSE MELANOMA ; EXPRESSION ; ADHESION ; CATENIN
英文摘要The epithelial-to-mesenchymal transition (EMT) plays crucial roles in embryonic development, wound healing, tissue repair, and cancer progression. Results of this study show how transforming growth factor beta 1 (TGF-beta 1) down-regulates expression of N-acetylglucosaminyltransferase III (GnT-III) during EMT-like changes. Treatment with TGF-beta 1 resulted in a decrease in E-cadherin expression and GnT-III expression, as well as its product, the bisected N-glycans, which was confirmed by erythro-agglutinating phytohemagglutinin lectin blot and HPLC analysis in human MCF-10A and mouse GE11 cells. In contrast with GnT-III, the expression of N-acetylglucosaminyltransferase V was slightly enhanced by TGF-beta 1 treatment. Changes in the N-glycan patterns on alpha 3 beta 1 integrin, one of the target proteins for GnT-III, were also confirmed by lectin blot analysis. To understand the roles of GnT-III expression in EMT-like changes, the MCF-10A cell was stably transfected with GnT-III. It is of particular interest that overexpression of GnT-III influenced EMT-like changes induced by TGF-beta 1, which was confirmed by cell morphological changes of phase contrast, immunochemical staining patterns of E-cadherin, and actin. In addition, GnT-III modified E-cadherin, which served to prolong E-cadherin turnover on the cell surface examined by biotinylation and pulse-chase experiments. GnT-III expression consistently inhibited beta-catenin translocation from cell-cell contact into the cytoplasm and nucleus. Furthermore, the transwell assay showed that GnT-III expression suppressed TGF-beta 1-induced cell motility. Taken together, these observations are the first to clearly demonstrate that GnT-III affects cell properties, which in turn influence EMT-like changes, and to explain a molecular mechanism for the inhibitory effects of GnT-III on cancer metastasis.
语种英语
WOS记录号WOS:000304030900046
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被引频次:51[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138064
专题中国科学院大连化学物理研究所
作者单位1.Tohoku Pharmaceut Univ, Inst Mol Biomembrane & Glycobiol, Div Regulatory Glycobiol, Aoba Ku, Sendai, Miyagi 9818558, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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Xu, Qingsong,Isaji, Tomoya,Lu, Yingying,et al. Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor beta 1 (TGF-beta 1) in Epithelial Cell Lines[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(20):16563-16574.
APA Xu, Qingsong.,Isaji, Tomoya.,Lu, Yingying.,Gu, Wei.,Kondo, Madoka.,...&Gu, Jianguo.(2012).Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor beta 1 (TGF-beta 1) in Epithelial Cell Lines.JOURNAL OF BIOLOGICAL CHEMISTRY,287(20),16563-16574.
MLA Xu, Qingsong,et al."Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor beta 1 (TGF-beta 1) in Epithelial Cell Lines".JOURNAL OF BIOLOGICAL CHEMISTRY 287.20(2012):16563-16574.
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