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题名: Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides
作者: Li, Qin1, 2;  Lynen, Frederic1;  Wang, Jian3;  Li, Hanlin3;  Xu, Guowang2;  Sandra, Pat1
关键词: Comprehensive two-dimensional liquid chromatography ;  Hydrophilic interaction liquid chromatography ;  Ion-pair reversed-phase liquid chromatography ;  LC x LC interface ;  Oligonucleotides
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2012-09-14
DOI: 10.1016/j.chroma.2011.11.062
卷: 1255, 页:237-243
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A comprehensive two-dimensional HPLC approach with a high degree of orthogonality was developed for analysis of di- to deca-oligonucleotides (ONs). Hydrophilic interaction liquid chromatography (HILIC) was used in the first dimension, and ion-pair reversed-phase liquid chromatography (IP-RPLC) was employed in the second dimension. The two dimensions were connected via a ten-port valve interface equipped with octadecyl silica (ODS) traps to immobilize and focus the ONs eluting from the first dimension prior to IP-RPLC separation. An aqueous make-up flow was used for effective trapping. The comprehensive two-dimensional HPLC system was optimized with a mixture consisting of 27 oligonucleotide standards. An overall chromatographic peak capacity of 500 was obtained. The use of the volatile buffer triethylamine acetate in the second dimension allowed straightforward coupling to electrospray ionization mass spectrometry (ESI-MS) and detection of each ON in the negative ionization mode. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: ANION-EXCHANGE CHROMATOGRAPHY ;  CAPILLARY GEL-ELECTROPHORESIS ;  MASS-SPECTROMETRY ;  PHOSPHOROTHIOATE OLIGONUCLEOTIDES ;  BIOLOGICAL-FLUIDS ;  INTERFERING RNA ;  NUCLEIC-ACIDS ;  PERFORMANCE ;  SEPARATION ;  PURIFICATION
语种: 英语
WOS记录号: WOS:000308524600024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138086
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Ghent, Pfizer Analyt Res Centre, B-9000 Ghent, Belgium
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Pfizer Inc, Pharmaceut Sci, Groton, CT 06340 USA

Recommended Citation:
Li, Qin,Lynen, Frederic,Wang, Jian,et al. Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1255:237-243.
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