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Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides
Li, Qin1,2; Lynen, Frederic1; Wang, Jian3; Li, Hanlin3; Xu, Guowang2; Sandra, Pat1
KeywordComprehensive Two-dimensional Liquid Chromatography Hydrophilic Interaction Liquid Chromatography Ion-pair Reversed-phase Liquid Chromatography Lc x Lc Interface Oligonucleotides
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2012-09-14
DOI10.1016/j.chroma.2011.11.062
Volume1255Pages:237-243
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordANION-EXCHANGE CHROMATOGRAPHY ; CAPILLARY GEL-ELECTROPHORESIS ; MASS-SPECTROMETRY ; PHOSPHOROTHIOATE OLIGONUCLEOTIDES ; BIOLOGICAL-FLUIDS ; INTERFERING RNA ; NUCLEIC-ACIDS ; PERFORMANCE ; SEPARATION ; PURIFICATION
AbstractA comprehensive two-dimensional HPLC approach with a high degree of orthogonality was developed for analysis of di- to deca-oligonucleotides (ONs). Hydrophilic interaction liquid chromatography (HILIC) was used in the first dimension, and ion-pair reversed-phase liquid chromatography (IP-RPLC) was employed in the second dimension. The two dimensions were connected via a ten-port valve interface equipped with octadecyl silica (ODS) traps to immobilize and focus the ONs eluting from the first dimension prior to IP-RPLC separation. An aqueous make-up flow was used for effective trapping. The comprehensive two-dimensional HPLC system was optimized with a mixture consisting of 27 oligonucleotide standards. An overall chromatographic peak capacity of 500 was obtained. The use of the volatile buffer triethylamine acetate in the second dimension allowed straightforward coupling to electrospray ionization mass spectrometry (ESI-MS) and detection of each ON in the negative ionization mode. (C) 2011 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000308524600024
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138086
Collection中国科学院大连化学物理研究所
Affiliation1.Univ Ghent, Pfizer Analyt Res Centre, B-9000 Ghent, Belgium
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Pfizer Inc, Pharmaceut Sci, Groton, CT 06340 USA
Recommended Citation
GB/T 7714
Li, Qin,Lynen, Frederic,Wang, Jian,et al. Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1255:237-243.
APA Li, Qin,Lynen, Frederic,Wang, Jian,Li, Hanlin,Xu, Guowang,&Sandra, Pat.(2012).Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides.JOURNAL OF CHROMATOGRAPHY A,1255,237-243.
MLA Li, Qin,et al."Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides".JOURNAL OF CHROMATOGRAPHY A 1255(2012):237-243.
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