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题名: Nano-graphene-based tyrosinase biosensor for rapid detection of bisphenol A
作者: Wu, Lidong1;  Deng, Dehui1;  Jin, Jing1;  Lu, Xianbo1;  Chen, Jiping1
关键词: Bisphenol A ;  Nanographene ;  Graphene ;  Carbon nanotubes ;  Tyrosinase biosensor
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2012-05-15
DOI: 10.1016/j.bios.2012.02.045
卷: 35, 期:1, 页:193-199
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: Hydrophilic nanographene (NGP) prepared by ball milling of graphite was used as the support to construct a novel tyrosinase biosensor for determination of bisphenol A (BPA). The performances of the nanographene-based tyrosinase biosensor were systematically compared with those of multiwall carbon nanotubes (MWNTs) modified tyrosinase biosensors. The results indicated that the nanographene-based tyrosinase biosensor provided significant advantages over MWNTs-based tyrosinase biosensor in term of response, repeatability, background current and limit of detection (LOD), which could be attributed to its larger specific surface area and unique hierarchical tyrosinase-NGP nanostructures. The nanographene-based tyrosinase biosensor displayed superior analytical performance over a linear range from 100 nmol L-1 to 2000 nmol L-1, with LOD of 33 nmol L-1 and sensitivity of 3108.4 mA cm(-2) M-1. The biosensor was further used for detecting BPA (leaching from different vessels) in tap water, and the accuracy of the results was validated by high performance liquid chromatography (HPLC). The nanographene-based tyrosinase biosensor proved to be a promising and reliable tool for rapid detection of BPA leached from polycarbonate plastic products and for on-site rapid analysis of emergency pollution affairs of BPA. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: ELECTROCHEMICAL BIOSENSORS ;  SELECTIVE DETECTION ;  FABRICATION ;  IMMOBILIZATION ;  DISORDERS ;  GLUCOSE ;  FILMS
语种: 英语
WOS记录号: WOS:000305036000029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138098
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Wu, Lidong,Deng, Dehui,Jin, Jing,et al. Nano-graphene-based tyrosinase biosensor for rapid detection of bisphenol A[J]. BIOSENSORS & BIOELECTRONICS,2012,35(1):193-199.
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