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题名: Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography
作者: Wang, HL;  Zou, HF;  Zhang, YK
关键词: microdialysis ;  fenoprofen ;  human serum albumin ;  multi-site binding ;  high performance liquid chromatography ;  competitive interaction
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 1998
卷: 12, 期:1, 页:4-7
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Pharmacology & Pharmacy
英文摘要: A simple and fast method for the determination of the multi-site binding of fenoprofen (FP) to human serum albumin (HSA) has been developed by utilizing microdialysis sampling techniques combined with high performance liquid chromatography (HPLC), The drug and protein were mixed in different molar ratios in 0.067 Mol potassium phosphate buffer, pH 7.4, and incubated at 37 degrees C in a water-bath. Then the microdialysis probe was put in the FP-HSA solution and sampled at the perfusion rate of 1 mu L/min. The concentrations of FP in microdialysates were determined by the reversed-phase high performance liquid chromatography, Relative recovery (R) was also determined in vitro on similar condition, R is about 56.03+/-1.11% (n=3). Fenoprofen was found to bind to two classes of sites, the association constant (K-1) and the number of the binding sites on primary binding sites of a HSA molecule (n(1)) for fenoprofen are 3.4 x 10(5)/M and 2.5, respectively, and those for secondary binding are 1.0 x 10(4)/M and 10.0, respectively, The competitive interaction of ibuprofen (IF) and palmitic acid with fenoprofen to HSA were also studied, both compounds significantly decrease the binding degree of fenoprofen to HSA. (C) 1998 John Wiley & Sons, Ltd.
关键词[WOS]: PLASMA-PROTEIN BINDING ;  FRONTAL ANALYSIS ;  CAPILLARY ELECTROPHORESIS ;  STATIONARY PHASE ;  SILICA COLUMN ;  DRUGS ;  ACID ;  CARBAMAZEPINE
语种: 英语
WOS记录号: WOS:000071630400002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138166
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Wang, HL,Zou, HF,Zhang, YK. Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography[J]. BIOMEDICAL CHROMATOGRAPHY,1998,12(1):4-7.
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