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Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography
Wang, HL; Zou, HF; Zhang, YK
KeywordMicrodialysis Fenoprofen Human Serum Albumin Multi-site Binding High Performance Liquid Chromatography Competitive Interaction
Source PublicationBIOMEDICAL CHROMATOGRAPHY
1998
Volume12Issue:1Pages:4-7
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS KeywordPLASMA-PROTEIN BINDING ; FRONTAL ANALYSIS ; CAPILLARY ELECTROPHORESIS ; STATIONARY PHASE ; SILICA COLUMN ; DRUGS ; ACID ; CARBAMAZEPINE
AbstractA simple and fast method for the determination of the multi-site binding of fenoprofen (FP) to human serum albumin (HSA) has been developed by utilizing microdialysis sampling techniques combined with high performance liquid chromatography (HPLC), The drug and protein were mixed in different molar ratios in 0.067 Mol potassium phosphate buffer, pH 7.4, and incubated at 37 degrees C in a water-bath. Then the microdialysis probe was put in the FP-HSA solution and sampled at the perfusion rate of 1 mu L/min. The concentrations of FP in microdialysates were determined by the reversed-phase high performance liquid chromatography, Relative recovery (R) was also determined in vitro on similar condition, R is about 56.03+/-1.11% (n=3). Fenoprofen was found to bind to two classes of sites, the association constant (K-1) and the number of the binding sites on primary binding sites of a HSA molecule (n(1)) for fenoprofen are 3.4 x 10(5)/M and 2.5, respectively, and those for secondary binding are 1.0 x 10(4)/M and 10.0, respectively, The competitive interaction of ibuprofen (IF) and palmitic acid with fenoprofen to HSA were also studied, both compounds significantly decrease the binding degree of fenoprofen to HSA. (C) 1998 John Wiley & Sons, Ltd.
Language英语
WOS IDWOS:000071630400002
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138166
Collection中国科学院大连化学物理研究所
AffiliationAcad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Wang, HL,Zou, HF,Zhang, YK. Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography[J]. BIOMEDICAL CHROMATOGRAPHY,1998,12(1):4-7.
APA Wang, HL,Zou, HF,&Zhang, YK.(1998).Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography.BIOMEDICAL CHROMATOGRAPHY,12(1),4-7.
MLA Wang, HL,et al."Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography".BIOMEDICAL CHROMATOGRAPHY 12.1(1998):4-7.
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