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Application of capillary zone electrophoresis to the study of interactions between Bacillus subtilis tryptophanyl-tRNA synthetase and tRNA(TrP)
Zhang, B; Xue, H; Lin, BC
KeywordCapillary Zone Electrophoresis Bacillus Subtilis Tryptophanyl-trna Synthetase
Source PublicationCHROMATOGRAPHIA
1998-08-01
Volume48Issue:3-4Pages:268-272
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPROTEIN-BINDING ; DRUG ; SEPARATION
AbstractThe application of capillary zone electrophoresis to the study of interactions between Bacillus subtilis tryptophanyl-tRNA synthetase (TrpRS) and tRNA(Trp) is described. Significant changes in peak shape of tRNA(Trp) incubated with TrpRS indicated the occurrence of interactions between TrpRS and tRNA(Trp) in pH 8.0 Tris-HCl buffer containing 0.1 mmol L-1 EDTA and 1 mmol L-1 - 5 mmol L-1 MgCl2. Addition of Mg2+ decreased the electrophoretic mobility of tRNA(Trp), which illustrated that conformation of tRNA(Trp) depended on Mg2+. The dissociation constant of the TrpRS-tRNA(Trp) complex was estimated to be 0.63 mu mol L-l at 25 degrees C in buffer solution.
Language英语
WOS IDWOS:000078156100014
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138172
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong
Recommended Citation
GB/T 7714
Zhang, B,Xue, H,Lin, BC. Application of capillary zone electrophoresis to the study of interactions between Bacillus subtilis tryptophanyl-tRNA synthetase and tRNA(TrP)[J]. CHROMATOGRAPHIA,1998,48(3-4):268-272.
APA Zhang, B,Xue, H,&Lin, BC.(1998).Application of capillary zone electrophoresis to the study of interactions between Bacillus subtilis tryptophanyl-tRNA synthetase and tRNA(TrP).CHROMATOGRAPHIA,48(3-4),268-272.
MLA Zhang, B,et al."Application of capillary zone electrophoresis to the study of interactions between Bacillus subtilis tryptophanyl-tRNA synthetase and tRNA(TrP)".CHROMATOGRAPHIA 48.3-4(1998):268-272.
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