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题名: Application of capillary zone electrophoresis to the study of interactions between Bacillus subtilis tryptophanyl-tRNA synthetase and tRNA(TrP)
作者: Zhang, B;  Xue, H;  Lin, BC
关键词: capillary zone electrophoresis ;  Bacillus subtilis ;  tryptophanyl-tRNA synthetase
刊名: CHROMATOGRAPHIA
发表日期: 1998-08-01
卷: 48, 期:3-4, 页:268-272
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The application of capillary zone electrophoresis to the study of interactions between Bacillus subtilis tryptophanyl-tRNA synthetase (TrpRS) and tRNA(Trp) is described. Significant changes in peak shape of tRNA(Trp) incubated with TrpRS indicated the occurrence of interactions between TrpRS and tRNA(Trp) in pH 8.0 Tris-HCl buffer containing 0.1 mmol L-1 EDTA and 1 mmol L-1 - 5 mmol L-1 MgCl2. Addition of Mg2+ decreased the electrophoretic mobility of tRNA(Trp), which illustrated that conformation of tRNA(Trp) depended on Mg2+. The dissociation constant of the TrpRS-tRNA(Trp) complex was estimated to be 0.63 mu mol L-l at 25 degrees C in buffer solution.
关键词[WOS]: PROTEIN-BINDING ;  DRUG ;  SEPARATION
语种: 英语
WOS记录号: WOS:000078156100014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138172
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong

Recommended Citation:
Zhang, B,Xue, H,Lin, BC. Application of capillary zone electrophoresis to the study of interactions between Bacillus subtilis tryptophanyl-tRNA synthetase and tRNA(TrP)[J]. CHROMATOGRAPHIA,1998,48(3-4):268-272.
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