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题名: Mannitol influence on the separation of DNA fragments by capillary electrophoresis in entangled polymer solutions
作者: Han, FT;  Xue, J;  Lin, BC
关键词: capillary electrophoresis ;  DNA separation ;  mannitol
刊名: TALANTA
发表日期: 1998-08-01
卷: 46, 期:4, 页:735-742
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A new kind of sieving matrix is presented in this paper to allow satisfactory separation of DNA fragments in a relatively low viscous solution. When a certain amount of mannitol was added to cellulose solution not concentrated enough to separate PGEM-3Zf(+)/HaeIII standards well, a polymer solution with low viscosity but with very good separation effects was obtained. The separation result of this sieving buffer was comparable with those using highly concentrated cellulose solutions. The sieving ability of solutions with different cellulose concentrations and different amounts of mannitol has been investigated. It was proved that 0.5% was the minimum hydroxypropylmethylcellulose (HPMC) concentration that could be used to separate DNA fragments satisfactorily. HPMC solutions with a concentration of less than 0.5% could not separate the standard DNA fragments even in the presence of mannitol. It was found that 6% was the optimized mannitol concentration because either more or less mannitol will lead a decrease of resolution. The principle of the positive influence of mannitol has also been discussed. (C) 1998 Elsevier Science B.V. All rights reserved.
关键词[WOS]: POLYMORPHISM ANALYSIS ;  CHAIN-REACTION ;  RESTRICTION FRAGMENTS ;  PRODUCTS
语种: 英语
WOS记录号: WOS:000074745200033
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138193
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Han, FT,Xue, J,Lin, BC. Mannitol influence on the separation of DNA fragments by capillary electrophoresis in entangled polymer solutions[J]. TALANTA,1998,46(4):735-742.
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