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Membrane supports as the stationary phase in high performance immunoaffinity chromatography
Zhou, DM; Zou, HF; Ni, JY; Yang, L; Jia, LY; Zhang, Q; Zhang, YK
刊名ANALYTICAL CHEMISTRY
1999
71期:1页:115-118
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PERFUSION CHROMATOGRAPHY ; AFFINITY-CHROMATOGRAPHY ; ION-EXCHANGE ; PURIFICATION ; PROTEINS ; SEPARATION ; ANTIBODY
英文摘要The membrane with a composite of cellulose grafted with acrylic polymers formed by polymerizing a glycidyl methacrylate in the presence of dispersed cellulose fiber was prepared as the stationary phase; the column (40 x 4 mm i.d.) which was compatible with the HPLC instrument was packed with pieces of the cut membrane. Protein A and human IgG were immobilized on the membrane stationary phase. The column based on the membrane support provided us good reproducibility, high efficiency, and low back pressure. High-performance immunoaffinity chromatographic analysis of human IgG in serum and polyclonal antibody to human IgG raised in goat was performed within 2.5 min. The fast-speed immunoaffinity analysis was developed by increasing the now rate of the mobile phase and decreasing the duration time for the switch of the mobile phase; an operation for immunoaffinity analysis of human IgG could be finished within 30 s.
语种英语
WOS记录号WOS:000077925500026
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被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138205
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Zhou, DM,Zou, HF,Ni, JY,et al. Membrane supports as the stationary phase in high performance immunoaffinity chromatography[J]. ANALYTICAL CHEMISTRY,1999,71(1):115-118.
APA Zhou, DM.,Zou, HF.,Ni, JY.,Yang, L.,Jia, LY.,...&Zhang, YK.(1999).Membrane supports as the stationary phase in high performance immunoaffinity chromatography.ANALYTICAL CHEMISTRY,71(1),115-118.
MLA Zhou, DM,et al."Membrane supports as the stationary phase in high performance immunoaffinity chromatography".ANALYTICAL CHEMISTRY 71.1(1999):115-118.
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