中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Membrane supports as the stationary phase in high performance immunoaffinity chromatography
作者: Zhou, DM;  Zou, HF;  Ni, JY;  Yang, L;  Jia, LY;  Zhang, Q;  Zhang, YK
刊名: ANALYTICAL CHEMISTRY
发表日期: 1999
卷: 71, 期:1, 页:115-118
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The membrane with a composite of cellulose grafted with acrylic polymers formed by polymerizing a glycidyl methacrylate in the presence of dispersed cellulose fiber was prepared as the stationary phase; the column (40 x 4 mm i.d.) which was compatible with the HPLC instrument was packed with pieces of the cut membrane. Protein A and human IgG were immobilized on the membrane stationary phase. The column based on the membrane support provided us good reproducibility, high efficiency, and low back pressure. High-performance immunoaffinity chromatographic analysis of human IgG in serum and polyclonal antibody to human IgG raised in goat was performed within 2.5 min. The fast-speed immunoaffinity analysis was developed by increasing the now rate of the mobile phase and decreasing the duration time for the switch of the mobile phase; an operation for immunoaffinity analysis of human IgG could be finished within 30 s.
关键词[WOS]: PERFUSION CHROMATOGRAPHY ;  AFFINITY-CHROMATOGRAPHY ;  ION-EXCHANGE ;  PURIFICATION ;  PROTEINS ;  SEPARATION ;  ANTIBODY
语种: 英语
WOS记录号: WOS:000077925500026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138205
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhou, DM,Zou, HF,Ni, JY,et al. Membrane supports as the stationary phase in high performance immunoaffinity chromatography[J]. ANALYTICAL CHEMISTRY,1999,71(1):115-118.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhou, DM]'s Articles
 [Zou, HF]'s Articles
 [Ni, JY]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhou, DM]‘s Articles
 [Zou, HF]‘s Articles
 [Ni, JY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace