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题名: Using capillary electrophoresis to evaluate deoxyribonucleic acid annealing reaction
作者: He, XY;  Zhou, XM;  Lin, BC
关键词: capillary electrophoresis ;  deoxyribonucleic acid ;  annealing
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2003
卷: 31, 期:1, 页:10-13
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Capillary Electrophoresis was used to evaluate the annealing of two complemental single stranded deoxyribonucleic acid (DNA) qualitatively and quantitatively. The annealing efficiency can be observed from the electropherogram. The migration characteristic of the double stranded DNA was explained. The NaCl concentration in the annealing DNA product matrix was determined. In general the existence of NaCl can lead the sample to dilute, but this factor could be neglected when the concentration of NaCl, was < 20 mmol/L in our experimental conditions.
语种: 英语
WOS记录号: WOS:000180964300003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138247
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
He, XY,Zhou, XM,Lin, BC. Using capillary electrophoresis to evaluate deoxyribonucleic acid annealing reaction[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2003,31(1):10-13.
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