中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Effects of experimental parameters on the signal intensity of capillary electrophoresis electrospray ionization mass spectrometry in protein analysis
作者: Liang, Z;  Yang, Q;  Zhang, WB;  Zhang, LH;  Zhang, YK
关键词: capillary electrophoresis ;  electrospray ionization-mass spectrometry ;  interface conditions ;  proteins
刊名: CHROMATOGRAPHIA
发表日期: 2003-05-01
卷: 57, 期:9-10, 页:617-621
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In recent years, the hyphenation of capillary electrophoresis (CE) and electrospray ionization mass spectrometry (ESI-MS) has been widely used for the analysis of biological molecules with high efficiency and high accuracy However, the signal intensity of CE-ESI-MS is restricted by various parameters. This paper reports the effects on the CE-ESI-MS signal of the pH and the electrolyte concentration, the formic acid concentration of the sheath liquid, and the sheath liquid composition, using several proteins as samples. The study was performed systematically by experimental and theoretical analyses. The maximum signal intensity of three proteins (cytochrome c, insulin, bovine serum albumin) was attained with a pH 4.40 buffer containing 75 mM formic acid and 75 mM ammonium acetate. Investigation of the influence of the formic acid concentration in the sheath liquid (over the range of 0%-1%) on the ESI-MS signal of brovine serum albumin showed that the feasible amount of the formic acid in sheath liquid could improve the signal intensity of the sample ions. However, considerable bond broadening was observed that should be attributed mainly to column overloading, band spreading at the interface, and scanning data acquisition.
关键词[WOS]: INTERFACE ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000183320400008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138255
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Liang, Z,Yang, Q,Zhang, WB,et al. Effects of experimental parameters on the signal intensity of capillary electrophoresis electrospray ionization mass spectrometry in protein analysis[J]. CHROMATOGRAPHIA,2003,57(9-10):617-621.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Liang, Z]'s Articles
 [Yang, Q]'s Articles
 [Zhang, WB]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Liang, Z]‘s Articles
 [Yang, Q]‘s Articles
 [Zhang, WB]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace