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Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup
Zhang, P; Xu, GW; Xiong, JH; Zheng, YF; Shi, XZ; Yang, Q; Wei, FS
KeywordElectrophoresis Micro-column Solid-phase Extraction Sensitivity Enrichment Sample Pretreatment
Source PublicationJOURNAL OF SEPARATION SCIENCE
2003-11-01
DOI10.1002/jssc.200301502
Volume26Issue:17Pages:1527-1532
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; MEMBRANE PRECONCENTRATION ; ZONE-ELECTROPHORESIS ; BIOLOGICAL SAMPLES ; ONLINE PRECONCENTRATION ; QUANTITATIVE-ANALYSIS ; CE ; SEPARATION ; STACKING
AbstractIn this paper, a setup called micro-column solid phase extraction (MicroSPE) was developed to enhance the sensitivity of capillary electrophoresis. The setup consists of a manual pump, several insulin syringes, and a Teflon extraction cartridge. The cartridge was made of 2-4 mm irregular C-18 solid phase materials in a Teflon tube (375 mum ID) with glass fiber frits. Two pieces of capillary tubing were connected to both the ends of the cartridge. Using sulinclac-spiked urine as the test sample, the extraction and analytical conditions were investigated in detail. When 1 muL 70/30 methanol/water was applied as the elution solvent, satisfactory results could be obtained. The internal standard method was used to quantitatively determine the compound in urine. The detection limit for sulindac was 2.9 ng/mL and the enrichment factor reaches more than 260. The result illustrates that the MicroSPE setup has a significant concentrating effect and is suitable for preconcentrating and cleaning-up samples with complex matrices.
Language英语
WOS IDWOS:000186719700009
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138257
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116011, Peoples R China
2.China Natl Environm Monitoring Ctr, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, P,Xu, GW,Xiong, JH,et al. Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(17):1527-1532.
APA Zhang, P.,Xu, GW.,Xiong, JH.,Zheng, YF.,Shi, XZ.,...&Wei, FS.(2003).Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup.JOURNAL OF SEPARATION SCIENCE,26(17),1527-1532.
MLA Zhang, P,et al."Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup".JOURNAL OF SEPARATION SCIENCE 26.17(2003):1527-1532.
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