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题名: Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup
作者: Zhang, P;  Xu, GW;  Xiong, JH;  Zheng, YF;  Shi, XZ;  Yang, Q;  Wei, FS
关键词: electrophoresis ;  micro-column solid-phase extraction ;  sensitivity ;  enrichment ;  sample ;  pretreatment
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2003-11-01
DOI: 10.1002/jssc.200301502
卷: 26, 期:17, 页:1527-1532
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: In this paper, a setup called micro-column solid phase extraction (MicroSPE) was developed to enhance the sensitivity of capillary electrophoresis. The setup consists of a manual pump, several insulin syringes, and a Teflon extraction cartridge. The cartridge was made of 2-4 mm irregular C-18 solid phase materials in a Teflon tube (375 mum ID) with glass fiber frits. Two pieces of capillary tubing were connected to both the ends of the cartridge. Using sulinclac-spiked urine as the test sample, the extraction and analytical conditions were investigated in detail. When 1 muL 70/30 methanol/water was applied as the elution solvent, satisfactory results could be obtained. The internal standard method was used to quantitatively determine the compound in urine. The detection limit for sulindac was 2.9 ng/mL and the enrichment factor reaches more than 260. The result illustrates that the MicroSPE setup has a significant concentrating effect and is suitable for preconcentrating and cleaning-up samples with complex matrices.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  MEMBRANE PRECONCENTRATION ;  ZONE-ELECTROPHORESIS ;  BIOLOGICAL SAMPLES ;  ONLINE PRECONCENTRATION ;  QUANTITATIVE-ANALYSIS ;  CE ;  SEPARATION ;  STACKING
语种: 英语
WOS记录号: WOS:000186719700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138257
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116011, Peoples R China
2.China Natl Environm Monitoring Ctr, Beijing, Peoples R China

Recommended Citation:
Zhang, P,Xu, GW,Xiong, JH,et al. Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(17):1527-1532.
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