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Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup
Zhang, P; Xu, GW; Xiong, JH; Zheng, YF; Shi, XZ; Yang, Q; Wei, FS
关键词Electrophoresis Micro-column Solid-phase Extraction Sensitivity Enrichment Sample Pretreatment
刊名JOURNAL OF SEPARATION SCIENCE
2003-11-01
DOI10.1002/jssc.200301502
26期:17页:1527-1532
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; MEMBRANE PRECONCENTRATION ; ZONE-ELECTROPHORESIS ; BIOLOGICAL SAMPLES ; ONLINE PRECONCENTRATION ; QUANTITATIVE-ANALYSIS ; CE ; SEPARATION ; STACKING
英文摘要In this paper, a setup called micro-column solid phase extraction (MicroSPE) was developed to enhance the sensitivity of capillary electrophoresis. The setup consists of a manual pump, several insulin syringes, and a Teflon extraction cartridge. The cartridge was made of 2-4 mm irregular C-18 solid phase materials in a Teflon tube (375 mum ID) with glass fiber frits. Two pieces of capillary tubing were connected to both the ends of the cartridge. Using sulinclac-spiked urine as the test sample, the extraction and analytical conditions were investigated in detail. When 1 muL 70/30 methanol/water was applied as the elution solvent, satisfactory results could be obtained. The internal standard method was used to quantitatively determine the compound in urine. The detection limit for sulindac was 2.9 ng/mL and the enrichment factor reaches more than 260. The result illustrates that the MicroSPE setup has a significant concentrating effect and is suitable for preconcentrating and cleaning-up samples with complex matrices.
语种英语
WOS记录号WOS:000186719700009
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138257
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116011, Peoples R China
2.China Natl Environm Monitoring Ctr, Beijing, Peoples R China
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Zhang, P,Xu, GW,Xiong, JH,et al. Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup[J]. JOURNAL OF SEPARATION SCIENCE,2003,26(17):1527-1532.
APA Zhang, P.,Xu, GW.,Xiong, JH.,Zheng, YF.,Shi, XZ.,...&Wei, FS.(2003).Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup.JOURNAL OF SEPARATION SCIENCE,26(17),1527-1532.
MLA Zhang, P,et al."Enhancing the sensitivity of capillary electrophoresis using a microcolumn solid phase extraction setup".JOURNAL OF SEPARATION SCIENCE 26.17(2003):1527-1532.
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