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Study on the interaction of metal ions in active center of nuclease P1 with CoCl2
Zheng, XF; Chi, NY; Zhang, QF; Yuan, YL; Li, XY; Hu, JH
关键词Nuclease P1 Cocl2 Nmr Interaction
刊名CHINESE JOURNAL OF INORGANIC CHEMISTRY
2003-04-01
19期:4页:377-380
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Inorganic & Nuclear
研究领域[WOS]Chemistry
关键词[WOS]PENICILLIUM-CITRINUM ; CRYSTAL-STRUCTURE ; SPECIFICITY ; RESOLUTION
英文摘要The paper using various ways, such as ICP, VIS, NMR and measurement of enzyme activity, extensively study the direct interaction of Nuclease P1 with inorganic metal. compound (CoCl2) . It has been found that in aqueous solution, there exists a direct interaction between the metal ions of the active center in the metalloenzyme (Nuclease PI) and external added CoCl2. As a result, part of the metal ions in metalloenzyme were substituted and the corresponding metalloenzyme derivatives were produced and the catalytic activity of enzyme were changed, and the H-1 NMR spectrum of Co (11)-substituted nuclease PI derivative of Co (11) in Zn2 site of active center was firstly obtained.
语种英语
WOS记录号WOS:000182036800008
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138352
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Coll Bioengn, Dalian 116622, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Zheng, XF,Chi, NY,Zhang, QF,et al. Study on the interaction of metal ions in active center of nuclease P1 with CoCl2[J]. CHINESE JOURNAL OF INORGANIC CHEMISTRY,2003,19(4):377-380.
APA Zheng, XF,Chi, NY,Zhang, QF,Yuan, YL,Li, XY,&Hu, JH.(2003).Study on the interaction of metal ions in active center of nuclease P1 with CoCl2.CHINESE JOURNAL OF INORGANIC CHEMISTRY,19(4),377-380.
MLA Zheng, XF,et al."Study on the interaction of metal ions in active center of nuclease P1 with CoCl2".CHINESE JOURNAL OF INORGANIC CHEMISTRY 19.4(2003):377-380.
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