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题名: Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine
作者: Mei, SR;  Yao, CH;  Cai, LS;  Xing, J;  Xu, GW;  Wu, CY
关键词: cancer ;  capillary electrophoresis ;  end-column amperometric detection ;  8-hydroxy-2 '-deoxyguanosine ;  urine
刊名: ELECTROPHORESIS
发表日期: 2003-05-01
卷: 24, 期:9, 页:1411-1415
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Urinary 8-hydroxy-2'-deoxyguanosine (80HdG) is an excellent marker of oxidative DNA damage. Until now, urinary 80HdG has been measured by high-performance liquid chromatography with electrochemical detection. A simple and sensitive method for the analysis of urinary 80HdG by capillary electrophoresis with end-column amperometric detection has been developed in our laboratory. A single-step solid-phase extraction procedure was optimized and used for extracting 80HdG from human urine. To improve the sensitivity of this method, a new focusing technique based on a dynamic pH junction was used. The limit of detection was 20 nm (signal-to-noise ratio S/N = 3), the linear range was 50 nm-10 mum, and the correlation coefficient was better than 0.999. The relative standard deviation (RSD) was found to be 0.57% for migration time, and 4.79% for peak current. To show the usefulness of the method, the urinary concentration of 80HdG in nine healthy persons and ten cancer patients was determined. The urinary concentration of 80HdG in cancer patients was significantly higher than that in healthy persons.
关键词[WOS]: OXIDATIVE DNA-DAMAGE ;  DYNAMIC PH JUNCTION ;  ELECTROCHEMICAL DETECTION ;  GAS-CHROMATOGRAPHY ;  8-HYDROXYDEOXYGUANOSINE ;  MUTATIONS ;  BIOMARKER ;  ASSAY ;  BASE
语种: 英语
WOS记录号: WOS:000182965000011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138353
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, R&A Ctr, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

Recommended Citation:
Mei, SR,Yao, CH,Cai, LS,et al. Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine[J]. ELECTROPHORESIS,2003,24(9):1411-1415.
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