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Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine
Mei, SR; Yao, CH; Cai, LS; Xing, J; Xu, GW; Wu, CY
关键词Cancer Capillary Electrophoresis End-column Amperometric Detection 8-hydroxy-2 '-deoxyguanosine Urine
刊名ELECTROPHORESIS
2003-05-01
24期:9页:1411-1415
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]OXIDATIVE DNA-DAMAGE ; DYNAMIC PH JUNCTION ; ELECTROCHEMICAL DETECTION ; GAS-CHROMATOGRAPHY ; 8-HYDROXYDEOXYGUANOSINE ; MUTATIONS ; BIOMARKER ; ASSAY ; BASE
英文摘要Urinary 8-hydroxy-2'-deoxyguanosine (80HdG) is an excellent marker of oxidative DNA damage. Until now, urinary 80HdG has been measured by high-performance liquid chromatography with electrochemical detection. A simple and sensitive method for the analysis of urinary 80HdG by capillary electrophoresis with end-column amperometric detection has been developed in our laboratory. A single-step solid-phase extraction procedure was optimized and used for extracting 80HdG from human urine. To improve the sensitivity of this method, a new focusing technique based on a dynamic pH junction was used. The limit of detection was 20 nm (signal-to-noise ratio S/N = 3), the linear range was 50 nm-10 mum, and the correlation coefficient was better than 0.999. The relative standard deviation (RSD) was found to be 0.57% for migration time, and 4.79% for peak current. To show the usefulness of the method, the urinary concentration of 80HdG in nine healthy persons and ten cancer patients was determined. The urinary concentration of 80HdG in cancer patients was significantly higher than that in healthy persons.
语种英语
WOS记录号WOS:000182965000011
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被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138353
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, R&A Ctr, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
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GB/T 7714
Mei, SR,Yao, CH,Cai, LS,et al. Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine[J]. ELECTROPHORESIS,2003,24(9):1411-1415.
APA Mei, SR,Yao, CH,Cai, LS,Xing, J,Xu, GW,&Wu, CY.(2003).Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine.ELECTROPHORESIS,24(9),1411-1415.
MLA Mei, SR,et al."Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine".ELECTROPHORESIS 24.9(2003):1411-1415.
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