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Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine
Mei, SR; Yao, CH; Cai, LS; Xing, J; Xu, GW; Wu, CY
KeywordCancer Capillary Electrophoresis End-column Amperometric Detection 8-hydroxy-2 '-deoxyguanosine Urine
Source PublicationELECTROPHORESIS
2003-05-01
Volume24Issue:9Pages:1411-1415
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordOXIDATIVE DNA-DAMAGE ; DYNAMIC PH JUNCTION ; ELECTROCHEMICAL DETECTION ; GAS-CHROMATOGRAPHY ; 8-HYDROXYDEOXYGUANOSINE ; MUTATIONS ; BIOMARKER ; ASSAY ; BASE
AbstractUrinary 8-hydroxy-2'-deoxyguanosine (80HdG) is an excellent marker of oxidative DNA damage. Until now, urinary 80HdG has been measured by high-performance liquid chromatography with electrochemical detection. A simple and sensitive method for the analysis of urinary 80HdG by capillary electrophoresis with end-column amperometric detection has been developed in our laboratory. A single-step solid-phase extraction procedure was optimized and used for extracting 80HdG from human urine. To improve the sensitivity of this method, a new focusing technique based on a dynamic pH junction was used. The limit of detection was 20 nm (signal-to-noise ratio S/N = 3), the linear range was 50 nm-10 mum, and the correlation coefficient was better than 0.999. The relative standard deviation (RSD) was found to be 0.57% for migration time, and 4.79% for peak current. To show the usefulness of the method, the urinary concentration of 80HdG in nine healthy persons and ten cancer patients was determined. The urinary concentration of 80HdG in cancer patients was significantly higher than that in healthy persons.
Language英语
WOS IDWOS:000182965000011
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138353
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, R&A Ctr, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Mei, SR,Yao, CH,Cai, LS,et al. Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine[J]. ELECTROPHORESIS,2003,24(9):1411-1415.
APA Mei, SR,Yao, CH,Cai, LS,Xing, J,Xu, GW,&Wu, CY.(2003).Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine.ELECTROPHORESIS,24(9),1411-1415.
MLA Mei, SR,et al."Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine".ELECTROPHORESIS 24.9(2003):1411-1415.
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