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题名: Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions
作者: Zheng, XF;  Wu, L;  Gao, DB;  Chi, NY;  Lin, QS;  Hu, JH
关键词: EPR spectroscopy ;  metalloenzyme ;  interaction ;  Cu(II)
刊名: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
发表日期: 2003-06-01
卷: 59, 期:8, 页:1751-1755
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Spectroscopy
研究领域[WOS]: Spectroscopy
英文摘要: In the past. the method of reconstitution was used to investigate the interaction between metalloenzymes (containing Zn(11)) and metal ions. In this paper, electron paramagnetic resonance (EPR) has been employed to firstly study the direct interactions between Bacillus subtilis neutral proteinase (BSNP), nuclease PI and Cu(II) ions added in aqueous solution, respectively. These results show that a dynamic equilibrium exists between the Zn(II) in the active site of native enzymes and the added Cu(II), the added Cu(II) partly replaces the Zn(II), forming Cu(II)-enzyme derivatives. As a result, the activity of the native enzymes is influenced. The influences of pH value on this kind of interaction have also been investigated, and the results demonstrate that the change of pH value has little influence on the system of nuclease P1, but has remarkable influence on BSNP. We firstly obtained the EPR spectra for Cu(II)-enzyme derivatives. In addition, the derivative of Cu(II)-BSNP exists in the solution with two different conformations (I type g(parallel to) = 2.34, A(parallel to) (mT) = 13.4; 11 type g(parallel to) = 2.25, A(parallel to) (mT) = 16.1), and this two conformations exchanged each other depending on pH. (C) 2002 Elsevier Science B.V. All rights reserved.
语种: 英语
WOS记录号: WOS:000183006700011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138378
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Coll Bioengn, Dalian 116627, Peoples R China
2.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
3.Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zheng, XF,Wu, L,Gao, DB,et al. Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2003,59(8):1751-1755.
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