DICP OpenIR
Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions
Zheng, XF; Wu, L; Gao, DB; Chi, NY; Lin, QS; Hu, JH
关键词Epr Spectroscopy Metalloenzyme Interaction Cu(Ii)
刊名SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
2003-06-01
59期:8页:1751-1755
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Spectroscopy
研究领域[WOS]Spectroscopy
英文摘要In the past. the method of reconstitution was used to investigate the interaction between metalloenzymes (containing Zn(11)) and metal ions. In this paper, electron paramagnetic resonance (EPR) has been employed to firstly study the direct interactions between Bacillus subtilis neutral proteinase (BSNP), nuclease PI and Cu(II) ions added in aqueous solution, respectively. These results show that a dynamic equilibrium exists between the Zn(II) in the active site of native enzymes and the added Cu(II), the added Cu(II) partly replaces the Zn(II), forming Cu(II)-enzyme derivatives. As a result, the activity of the native enzymes is influenced. The influences of pH value on this kind of interaction have also been investigated, and the results demonstrate that the change of pH value has little influence on the system of nuclease P1, but has remarkable influence on BSNP. We firstly obtained the EPR spectra for Cu(II)-enzyme derivatives. In addition, the derivative of Cu(II)-BSNP exists in the solution with two different conformations (I type g(parallel to) = 2.34, A(parallel to) (mT) = 13.4; 11 type g(parallel to) = 2.25, A(parallel to) (mT) = 16.1), and this two conformations exchanged each other depending on pH. (C) 2002 Elsevier Science B.V. All rights reserved.
语种英语
WOS记录号WOS:000183006700011
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138378
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Coll Bioengn, Dalian 116627, Peoples R China
2.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
3.Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Zheng, XF,Wu, L,Gao, DB,et al. Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2003,59(8):1751-1755.
APA Zheng, XF,Wu, L,Gao, DB,Chi, NY,Lin, QS,&Hu, JH.(2003).Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,59(8),1751-1755.
MLA Zheng, XF,et al."Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 59.8(2003):1751-1755.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zheng, XF]的文章
[Wu, L]的文章
[Gao, DB]的文章
百度学术
百度学术中相似的文章
[Zheng, XF]的文章
[Wu, L]的文章
[Gao, DB]的文章
必应学术
必应学术中相似的文章
[Zheng, XF]的文章
[Wu, L]的文章
[Gao, DB]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。