DICP OpenIR
Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase
Ping, GC; Zhang, WB; Zhang, LH; Schmitt-Kopplin, P; Zhang, YK; Kettrup, A
KeywordCapillary Electrochromatography Monolithic Column Nucleosides
Source PublicationCHROMATOGRAPHIA
2003-05-01
Volume57Issue:9-10Pages:629-633
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; RIGID POLYMER MONOLITHS ; COLUMNS ; MEDIA
AbstractSeparation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic column is described. Because the nucleosides are relatively hydrophilic compounds, chromatographic partition is very weak on non-polar stationary phases. A small quantity of organic modifier is usually required to increase their retention factors but, because the wettability of the stationary phase by the mobile phase is rather poor, bubble formation can be a significant problem. This situation was improved by use of a polymeric monolithic column with higher level charged monomer in the polymerization mixture, because of the presence of charged moieties on the surface of the monolithic stationary phase. The very high stability under conditions of very low organic modifier concentration used in the experiment showed that the wettability of the stationary phase by the mobile phase was satisfactory. The effect of pH, organic modifier content, and applied voltage on the separation was investigated. Baseline separation of six nucleosides was achieved within 4.5 min on this type of column. Because of the presence of charged moieties and butyl groups on the surface of the monolithic column, the mechanism of separation might involve both ion-exchange and hydrophobic interactions.
Language英语
WOS IDWOS:000183320400010
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138390
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
2.GSF Natl Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany
Recommended Citation
GB/T 7714
Ping, GC,Zhang, WB,Zhang, LH,et al. Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase[J]. CHROMATOGRAPHIA,2003,57(9-10):629-633.
APA Ping, GC,Zhang, WB,Zhang, LH,Schmitt-Kopplin, P,Zhang, YK,&Kettrup, A.(2003).Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase.CHROMATOGRAPHIA,57(9-10),629-633.
MLA Ping, GC,et al."Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase".CHROMATOGRAPHIA 57.9-10(2003):629-633.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ping, GC]'s Articles
[Zhang, WB]'s Articles
[Zhang, LH]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ping, GC]'s Articles
[Zhang, WB]'s Articles
[Zhang, LH]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ping, GC]'s Articles
[Zhang, WB]'s Articles
[Zhang, LH]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.