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题名: Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase
作者: Ping, GC;  Zhang, WB;  Zhang, LH;  Schmitt-Kopplin, P;  Zhang, YK;  Kettrup, A
关键词: capillary electrochromatography ;  monolithic column ;  nucleosides
刊名: CHROMATOGRAPHIA
发表日期: 2003-05-01
卷: 57, 期:9-10, 页:629-633
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic column is described. Because the nucleosides are relatively hydrophilic compounds, chromatographic partition is very weak on non-polar stationary phases. A small quantity of organic modifier is usually required to increase their retention factors but, because the wettability of the stationary phase by the mobile phase is rather poor, bubble formation can be a significant problem. This situation was improved by use of a polymeric monolithic column with higher level charged monomer in the polymerization mixture, because of the presence of charged moieties on the surface of the monolithic stationary phase. The very high stability under conditions of very low organic modifier concentration used in the experiment showed that the wettability of the stationary phase by the mobile phase was satisfactory. The effect of pH, organic modifier content, and applied voltage on the separation was investigated. Baseline separation of six nucleosides was achieved within 4.5 min on this type of column. Because of the presence of charged moieties and butyl groups on the surface of the monolithic column, the mechanism of separation might involve both ion-exchange and hydrophobic interactions.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  RIGID POLYMER MONOLITHS ;  COLUMNS ;  MEDIA
语种: 英语
WOS记录号: WOS:000183320400010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138390
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
2.GSF Natl Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany

Recommended Citation:
Ping, GC,Zhang, WB,Zhang, LH,et al. Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase[J]. CHROMATOGRAPHIA,2003,57(9-10):629-633.
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