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Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers
Yang, L; Jia, LY; Zou, HF; Zhou, DM; Zhang, YK
关键词Membrane Affinity Chromatography Matrix Separation Biopolymers
刊名SCIENCE IN CHINA SERIES B-CHEMISTRY
1998-12-01
41期:6页:596-605
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]CHROMATOGRAPHY ; PURIFICATION ; PROTEINS
英文摘要A new iminodiacetic acid (IDA) type chelating membrane based on composite matrix formed by covalently coupling the polyglycidyl methacrylate to cotton cellulose fiber was developed. The study focused on the optimal conditions for the preparation of the membrane and the reaction of the chelating agent (iminodiacetic acid, IDA) with the matrix. The physical properties of the chelating membrane were tested by electron micrography and BET surface area instrument. The relationship between flow-rate and backpressure was almost linear. Adsorption isotherm of protein on Cu2+ - IDA membrane was determined by batch experiments. The dissociation constants and the maximum adsorption capacity were obtained. The results of affinity purification of human serum albumin and bovine liver catalase on membrane are comparable with those on gel chromatography presented in literature. The separations performed on the membrane chromatography, however, were 3-5 times faster than those on gel chromatography. The immobilized metal composite membrane was also successfully used in HPLC for rapid analysis of protein. Thus, the composite membrane reported here, with low backpressure, low cost and long service-life, is quite suitable for rapid and large scale affinity purification and HPLC analysis of biopolymers.
语种英语
WOS记录号WOS:000077599300005
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138470
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116012, Peoples R China
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GB/T 7714
Yang, L,Jia, LY,Zou, HF,et al. Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,1998,41(6):596-605.
APA Yang, L,Jia, LY,Zou, HF,Zhou, DM,&Zhang, YK.(1998).Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers.SCIENCE IN CHINA SERIES B-CHEMISTRY,41(6),596-605.
MLA Yang, L,et al."Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers".SCIENCE IN CHINA SERIES B-CHEMISTRY 41.6(1998):596-605.
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