DICP OpenIR
Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers
Yang, L; Jia, LY; Zou, HF; Zhou, DM; Zhang, YK
KeywordMembrane Affinity Chromatography Matrix Separation Biopolymers
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
1998-12-01
Volume41Issue:6Pages:596-605
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordCHROMATOGRAPHY ; PURIFICATION ; PROTEINS
AbstractA new iminodiacetic acid (IDA) type chelating membrane based on composite matrix formed by covalently coupling the polyglycidyl methacrylate to cotton cellulose fiber was developed. The study focused on the optimal conditions for the preparation of the membrane and the reaction of the chelating agent (iminodiacetic acid, IDA) with the matrix. The physical properties of the chelating membrane were tested by electron micrography and BET surface area instrument. The relationship between flow-rate and backpressure was almost linear. Adsorption isotherm of protein on Cu2+ - IDA membrane was determined by batch experiments. The dissociation constants and the maximum adsorption capacity were obtained. The results of affinity purification of human serum albumin and bovine liver catalase on membrane are comparable with those on gel chromatography presented in literature. The separations performed on the membrane chromatography, however, were 3-5 times faster than those on gel chromatography. The immobilized metal composite membrane was also successfully used in HPLC for rapid analysis of protein. Thus, the composite membrane reported here, with low backpressure, low cost and long service-life, is quite suitable for rapid and large scale affinity purification and HPLC analysis of biopolymers.
Language英语
WOS IDWOS:000077599300005
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138470
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116012, Peoples R China
Recommended Citation
GB/T 7714
Yang, L,Jia, LY,Zou, HF,et al. Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,1998,41(6):596-605.
APA Yang, L,Jia, LY,Zou, HF,Zhou, DM,&Zhang, YK.(1998).Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers.SCIENCE IN CHINA SERIES B-CHEMISTRY,41(6),596-605.
MLA Yang, L,et al."Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers".SCIENCE IN CHINA SERIES B-CHEMISTRY 41.6(1998):596-605.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, L]'s Articles
[Jia, LY]'s Articles
[Zou, HF]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, L]'s Articles
[Jia, LY]'s Articles
[Zou, HF]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, L]'s Articles
[Jia, LY]'s Articles
[Zou, HF]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.