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题名: Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers
作者: Yang, L;  Jia, LY;  Zou, HF;  Zhou, DM;  Zhang, YK
关键词: membrane ;  affinity chromatography ;  matrix ;  separation ;  biopolymers
刊名: SCIENCE IN CHINA SERIES B-CHEMISTRY
发表日期: 1998-12-01
卷: 41, 期:6, 页:596-605
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A new iminodiacetic acid (IDA) type chelating membrane based on composite matrix formed by covalently coupling the polyglycidyl methacrylate to cotton cellulose fiber was developed. The study focused on the optimal conditions for the preparation of the membrane and the reaction of the chelating agent (iminodiacetic acid, IDA) with the matrix. The physical properties of the chelating membrane were tested by electron micrography and BET surface area instrument. The relationship between flow-rate and backpressure was almost linear. Adsorption isotherm of protein on Cu2+ - IDA membrane was determined by batch experiments. The dissociation constants and the maximum adsorption capacity were obtained. The results of affinity purification of human serum albumin and bovine liver catalase on membrane are comparable with those on gel chromatography presented in literature. The separations performed on the membrane chromatography, however, were 3-5 times faster than those on gel chromatography. The immobilized metal composite membrane was also successfully used in HPLC for rapid analysis of protein. Thus, the composite membrane reported here, with low backpressure, low cost and long service-life, is quite suitable for rapid and large scale affinity purification and HPLC analysis of biopolymers.
关键词[WOS]: CHROMATOGRAPHY ;  PURIFICATION ;  PROTEINS
语种: 英语
WOS记录号: WOS:000077599300005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138470
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116012, Peoples R China

Recommended Citation:
Yang, L,Jia, LY,Zou, HF,et al. Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,1998,41(6):596-605.
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