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题名: Stereoselective binding of warfarin and ketoprofen to human serum albumin determined by microdialysis combined with HPLC
作者: Zou, HF;  Wang, HL;  Zhang, YK
刊名: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
发表日期: 1998
卷: 21, 期:17, 页:2663-2674
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Microdialysis sampling technique combined with high performance liquid chromatography (HPLC) was utilized to determine the stereoselective binding of warfarin and ketoprofen to human serum albumin (HSA) ii solution. The HPLC conditions for separation of warfarin aid ketoprofen enantiomers on HSA column were optimized. The unbound enantiomers in the mixed buffer (0.067 M phosphate and pH 7.4) solution of the drug racemate with HAS were sampled with microdialysis probe, and the amount of them was determined by HPLC on HSA column. It was observed that the unbound concentrations of R-warfarin and R-ketoprofen are about 1.08 similar to 1.34- and 1.08 similar to 1.15-fold of S-warfarin and S-ketoprofen, respectively, which indicated that the S-enantiomers bind to HSA more strongly than R-enantiomers to HSA. The interaction parameters for binding of warfarin and ketoprofen enantiomers to HSA including binding constants and number of binding site were obtained by the Scatchard analysis of experimental data, and the stereoselectivity (alpha) calculated by the binding constants of warfarin enantiomers to HSA is 1.92, but that of ketoprofen enantiomers is very close. HSA exhibits stronger stereoselectivity to warfarin racemate than to ketoprofen racemate.
关键词[WOS]: ENANTIOMERS ;  PHARMACOKINETICS ;  COLUMNS
语种: 英语
WOS记录号: WOS:000076379400007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138483
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zou, HF,Wang, HL,Zhang, YK. Stereoselective binding of warfarin and ketoprofen to human serum albumin determined by microdialysis combined with HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,1998,21(17):2663-2674.
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