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Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells
Zhang, W; Seki, M; Furusaki, S; Middelberg, APJ
关键词Strawberry Suspension Culture Anthocyanin Kinetics Nutrient Uptake Mathematical Model
刊名JOURNAL OF FERMENTATION AND BIOENGINEERING
1998
86期:1页:72-78
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Food Science & Technology
研究领域[WOS]Biotechnology & Applied Microbiology ; Food Science & Technology
关键词[WOS]VIABILITY ASSAY ; CARROT CELLS ; PLANT-CELLS ; VITIS SP ; IDENTIFICATION ; KINETICS ; CHLORIDE ; TISSUE ; MODEL ; SUGAR
英文摘要Strawberry (Fragaria ananassa cv. Shikinari) cell suspension cultures carried out in shake flasks for 18 d were closely examined for cell growth, anthocyanin synthesis and the development of pigmented cells in relation to the uptake of carbohydrate, extracellular PO4, NO3, NH4, and calcium. Cell viability, extracellular anthocyanin content, pH and electrical conductivity of the broth were also monitored. The specific growth rate of strawberry cells at exponential phase was 0.27 and 0.28 d(-1) based on fresh and dry weight, respectively. Anthocyanin synthesis was observed to increase continuously to a maximum value of 0.86 mg/g fresh cell weight (FCW) at day 6, and was partially growth-associated. Anthocyanin synthesis was linearly related to the increase in pigmented cell ratio, which increased with time and reached a maximum value of ca. 70% at day 6 due to reduction in cell viability and depletion of substrate. Total carbohydrate uptake was closely associated with increase in cell growth, and glucose was utilized in preference to fructose. Nitrate and ammonia were consumed until 9 d of culture, but phosphate was completely absorbed within 4 d. Calcium was assimilated throughout the growth cycle. After 9 d, cell lysis was observed which resulted in the leakage of intracellular substances and a concomitant pH rise. Anthocyanin was never detected in the broth although the broth became darkly pigmented during the lysis period. This suggests that anthocyanin was synthesized only by viable pigmented cells, and degraded rapidly upon cell death and lysis. Based on the results of kinetic analysis, a model was developed by incorporating governing equations for the ratio of pigmented cells into a Bailey and Nicholson's model. This was verified by comparison with the experimental data. The results suggest Bat the model satisfactorily describes the strawberry cell culture process, and may thus be used for process optimization.
语种英语
WOS记录号WOS:000075734500012
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138514
专题中国科学院大连化学物理研究所
作者单位1.Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biochem Engn, Dalian 116012, Peoples R China
3.Univ Adelaide, Dept Chem Engn, Adelaide, SA 5005, Australia
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Zhang, W,Seki, M,Furusaki, S,et al. Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells[J]. JOURNAL OF FERMENTATION AND BIOENGINEERING,1998,86(1):72-78.
APA Zhang, W,Seki, M,Furusaki, S,&Middelberg, APJ.(1998).Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells.JOURNAL OF FERMENTATION AND BIOENGINEERING,86(1),72-78.
MLA Zhang, W,et al."Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells".JOURNAL OF FERMENTATION AND BIOENGINEERING 86.1(1998):72-78.
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