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题名: Several problems in analysis of flavonoids from Ginkgo biloba by high performance liquid chromatography
作者: Wang, JD;  Xiong, BH;  Cong, RZ;  Shen, L
关键词: high performance liquid chromatography ;  Ginkgo biloba leaves ;  flavonoids
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 1999-09-01
卷: 27, 期:9, 页:1076-1079
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The operating conditions in several key links for analysis of contents of flavonoids from the leaves of Ginkgo biloba by high performance liquid chromatography( HPLC) have been investigated, They included the composition of hydrolysis solution, the temperature and the time of the hydrolysis. The possibility of using only one standard of quercetin to evaluate the contents of three flavonoids, i.e. quercetin, kaempferol and isorhamnetin at a wavelength of 370nm was demonstrated. The selected hydrolysis conditions were using of a 5.5%( W/V) HCl in methanol as hydrolysis solution at 85 similar to 90 degrees C oil bath where temperature hold slight boiling and to reflux for 1.3 hrs. HPLC conditions were Bio-Sil ODS,5 mu m, column, 150 x 4.0 mm I.D., MeOH/0.4%( V/V) phosphoric acid aquious solution(57: 43) as the mobile phase and detection at 370 nm. The satisfactory recovery and analytical accuracy can be obtained under these operating conditions.
关键词[WOS]: IDENTIFICATION
语种: 英语
WOS记录号: WOS:000083010500023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138531
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Wang, JD,Xiong, BH,Cong, RZ,et al. Several problems in analysis of flavonoids from Ginkgo biloba by high performance liquid chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,1999,27(9):1076-1079.
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