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题名: Study of physically adsorbed stationary phases for open tubular capillary electrochromatography
作者: Liu, Z;  Zou, HF;  Ye, ML;  Ni, JY;  Zhang, YK
关键词: open tubular capillary electrochromatography adsorption ;  protein ;  peptide ;  amino
刊名: ELECTROPHORESIS
发表日期: 1999-10-01
卷: 20, 期:14, 页:2891-2897
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel method based on the adsorption of positively charged compounds on the wall of a fused-silica capillary was applied to prepare stationary phases for open tubular capillary electrochromatography (OTCEC). The positively charged substances including cationic surfactant such as cetyltrimethylammonium bromide (CTAB) and basic chiral selectors such as protein, peptide and amino acid were physically adsorbed onto the capillary wall under specially selected conditions. The adsorbed stationary phase of CTAB was used to separate neutral compounds, while the others were used for chiral separations. The run-to-run reproducibility of retention time was rather good with relative standard deviation (RSD) values of less than 2.3%. The separation efficiency was excellent with the highest theoretical plate number of up to 590 000/m and the average one above 250 000/m. Stored at 2-8 degrees C in the refrigerator, the adsorbed stationary phase can last at least one month. It was observed that the UV spectra for the enantiomers are significantly different due to the diastereomeric interactions of enantiomers with the chiral stationary phase in the detection window. With the use of the same capillary, the same instrument, and the same mobile phase, the superiority of OTCEC over open tubular liquid chromatography (OTLC) and capillary zone electrophoresis (CZE) was illustrated.
关键词[WOS]: FUSED-SILICA CAPILLARIES ;  FLIGHT MASS DETECTOR ;  LIQUID-CHROMATOGRAPHY ;  ELECTROPHORETIC TECHNIQUES ;  ENANTIOMER SEPARATION ;  GRADIENT ELUTION ;  SELECTIVITY ;  RESOLUTION ;  FLOW ;  PERFORMANCE
语种: 英语
WOS记录号: WOS:000085044200016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138542
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Lab Chromatog, Dalian 116011, Peoples R China

Recommended Citation:
Liu, Z,Zou, HF,Ye, ML,et al. Study of physically adsorbed stationary phases for open tubular capillary electrochromatography[J]. ELECTROPHORESIS,1999,20(14):2891-2897.
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