DICP OpenIR
Gene diagnosis of phenylketonuria by capillary electrophoresis in a novel nongel sieving polymer solution
Han, F; Lin, B; Xu, Q; Shen, Y; Wu, G
KeywordCapillary Electrophoresis Phenylketonuria Gene Diagnosis
Source PublicationCHROMATOGRAPHIA
1999-02-01
Volume49Issue:3-4Pages:179-184
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPHENYLALANINE-HYDROXYLASE GENE ; DNA RESTRICTION FRAGMENTS ; PRENATAL-DIAGNOSIS ; SEPARATION ; RESOLUTION ; POLYMORPHISM ; PRODUCTS
AbstractIt is not possible to estimate DNA fragment lengths in undenatured buffer systems by the calibrating method because a conformation problem can occasionally predominate. Although some authors use urea as a denaturant to eliminate the influence of conformation on migration, they often experience the difficulty of loss of resolution. We have developed a novel sieving matrix with relatively low viscosity and good sieving ability, by which DNA standard PBR322/HaeIII could be separated satisfactorily. The influence of DNA conformation can also be eliminated in the same system: which makes it possible to estimate the length of a DNA fragment more precisely. Gene diagnosis and family linkage analysis of Phenylketonuria (PKU) was performed as an application. A four-base-pair difference in length of the phenylalanine hydroxylase (PAH) short-tandem repeat (STR) alleles could be separated and identified, which paved the way for diagnosis of PKU by capillary electrophoresis.
Language英语
WOS IDWOS:000078752700011
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138562
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Med Sci, Inst Basic Med, Beijing 100005, Peoples R China
Recommended Citation
GB/T 7714
Han, F,Lin, B,Xu, Q,et al. Gene diagnosis of phenylketonuria by capillary electrophoresis in a novel nongel sieving polymer solution[J]. CHROMATOGRAPHIA,1999,49(3-4):179-184.
APA Han, F,Lin, B,Xu, Q,Shen, Y,&Wu, G.(1999).Gene diagnosis of phenylketonuria by capillary electrophoresis in a novel nongel sieving polymer solution.CHROMATOGRAPHIA,49(3-4),179-184.
MLA Han, F,et al."Gene diagnosis of phenylketonuria by capillary electrophoresis in a novel nongel sieving polymer solution".CHROMATOGRAPHIA 49.3-4(1999):179-184.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Han, F]'s Articles
[Lin, B]'s Articles
[Xu, Q]'s Articles
Baidu academic
Similar articles in Baidu academic
[Han, F]'s Articles
[Lin, B]'s Articles
[Xu, Q]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Han, F]'s Articles
[Lin, B]'s Articles
[Xu, Q]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.