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题名: Application of capillary nongel sieving electrophoresis for gene analysis
作者: Shen, Y;  Xu, Q;  Han, FT;  Ding, K;  Song, F;  Fan, Y;  Zhu, N;  Wu, GY;  Lin, BC
关键词: capillary nongel electrophoresis ;  gene analysis
刊名: ELECTROPHORESIS
发表日期: 1999-07-01
卷: 20, 期:9, 页:1822-1828
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Capillary electrophoresis (CE) has proved to be a strong tool for DNA analysis and has found abundant applications in the fields of restriction fragment sizing, mutation screening, polymerase chain reaction (PCR) product characterizing and forensic identifying. CE may be the main alternative to slab gel electrophoresis. Capillary nongel electrophoresis is the most favorable mode when aiming for this purpose because of its advantages of long lifetime, easy operation, good reproducibility, and low expense. In this paper, a new kind of sieving matrix, with mannitol as the additive for capillary electrophoresis, as well as related methods and their application for gene analysis were reported. Nine DNA fragments amplified by multiplex PCR from a normal dystrophin gene were well separated by this system. Three different deletions were found in Duchenne muscular dystrophy (DMD) patients. Three to four copies of the sex-determination region of the Y chromosome (SRY) gene, as well as the phenylalanine hydroxylase (PAH) gene, could be detected in mixed samples. The frequencies of short tandem repeats (STR) in PAH genes was analyzed in 61 normal Chinese individuals and 6 phenylketonuria families. One case of prenatal gene diagnosis was performed. By using this matrix, CE coupled with reverse transcription PCR (RT-PCR), the analysis of the alternative splicing expression pattern of the fragile X mental retardation 1 (FMRI) gene in adult lung tissue was achieved.
关键词[WOS]: MICROSATELLITE INSTABILITY ;  PRENATAL-DIAGNOSIS ;  PHENYLKETONURIA ;  DISEASE ;  CANCER ;  CELLS ;  BLOOD ;  DNA
语种: 英语
WOS记录号: WOS:000081688800003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138572
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.CAMS & PUMC, Natl Lab Med Mol Biol, Inst Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100005, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Beijing, Peoples R China
3.Beijing Inst Pediat, Beijing, Peoples R China

Recommended Citation:
Shen, Y,Xu, Q,Han, FT,et al. Application of capillary nongel sieving electrophoresis for gene analysis[J]. ELECTROPHORESIS,1999,20(9):1822-1828.
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