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Study on the interaction of copper-zinc superoxide dismutase with cobalt(II)-histidine by spectral analysis - II. Effect of amount of external added Co(His)(n), phosphate
Zheng, XF; Hu, JH; Cheng, GB; Xu, YT; Zhao, YK; Liu, MH
关键词Cu2zn2sod Co(His)(n) Interaction
刊名SPECTROSCOPY AND SPECTRAL ANALYSIS
1999-12-01
19期:6页:798-802
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Spectroscopy
研究领域[WOS]Spectroscopy
英文摘要The effect of changing the amount of external added Co(His), and phosphate on the interaction of Cu2Zn2SOD with organic metal compound (Cobalt(II)-Histidine) have been studied by means of ICP,VIS and measurement of enzyme activity. It has found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2Zn2SOD) with external added Co(His)(n). As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co(II)-substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios (Cobalt(II)-Histidine) and of the presence of phosphate and got some results under the effect of corresponding factors.
语种英语
WOS记录号WOS:000084430400010
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138575
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China
2.Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Dalian Univ, Dept Chem & Chem Engn, Dalian 116622, Peoples R China
4.Liaoning Normal Univ, Expt Ctr, Dalian 116021, Peoples R China
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GB/T 7714
Zheng, XF,Hu, JH,Cheng, GB,et al. Study on the interaction of copper-zinc superoxide dismutase with cobalt(II)-histidine by spectral analysis - II. Effect of amount of external added Co(His)(n), phosphate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,1999,19(6):798-802.
APA Zheng, XF,Hu, JH,Cheng, GB,Xu, YT,Zhao, YK,&Liu, MH.(1999).Study on the interaction of copper-zinc superoxide dismutase with cobalt(II)-histidine by spectral analysis - II. Effect of amount of external added Co(His)(n), phosphate.SPECTROSCOPY AND SPECTRAL ANALYSIS,19(6),798-802.
MLA Zheng, XF,et al."Study on the interaction of copper-zinc superoxide dismutase with cobalt(II)-histidine by spectral analysis - II. Effect of amount of external added Co(His)(n), phosphate".SPECTROSCOPY AND SPECTRAL ANALYSIS 19.6(1999):798-802.
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