DICP OpenIR
Membrane affinity chromatography for analysis and purification of biopolymers
Zhou, DM; Zou, HF; Ni, JY; Wang, HL; Yang, L; Zhang, YK
关键词Column Liquid Chromatography Membrane Stationary Phase Immunoaffinity Analysis Affinity Efficiency Adsorption Capacity
刊名CHROMATOGRAPHIA
1999-07-01
50期:1-2页:27-34
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]BIOMOLECULES ; PERFORMANCE ; PROTEINS ; REMOVAL
英文摘要Affinity columns suitable for HPLC were prepared by immobilization of various ligands of protein A, human IgG, human IgM and pectinase on GMA modified cellulose membrane. The adsorption capacity, affinity efficiency and activity recovery of various IgGs on these affinity columns were measured. It was observed that the length of the coupling arm plays a very important role in affinity efficiency, and the effect of eluent flow-rate on adsorption capacity was very small. The protein A column was exploited for the process monitoring of dog IgG in clinical experiments on immuno-adsorption therapy. A pectinase column was used for the determination of polygalacturonase inhibiting proteins first purified on a hydroxyapatite column. It took only about 2.5 min for analysis at a flow-rate of 1.0 mL min(-1). The high speed analysis of biopolymers could be performed at a flow rate of 6.0 mL min(-1) within 15 s. Membrane affinity chromatography gives good reproducibility, high efficiency, low column-pressure and is rapid. It can also be used for micro-scale purification of biopolymers.
语种英语
WOS记录号WOS:000081720200006
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138576
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Zhou, DM,Zou, HF,Ni, JY,et al. Membrane affinity chromatography for analysis and purification of biopolymers[J]. CHROMATOGRAPHIA,1999,50(1-2):27-34.
APA Zhou, DM,Zou, HF,Ni, JY,Wang, HL,Yang, L,&Zhang, YK.(1999).Membrane affinity chromatography for analysis and purification of biopolymers.CHROMATOGRAPHIA,50(1-2),27-34.
MLA Zhou, DM,et al."Membrane affinity chromatography for analysis and purification of biopolymers".CHROMATOGRAPHIA 50.1-2(1999):27-34.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhou, DM]的文章
[Zou, HF]的文章
[Ni, JY]的文章
百度学术
百度学术中相似的文章
[Zhou, DM]的文章
[Zou, HF]的文章
[Ni, JY]的文章
必应学术
必应学术中相似的文章
[Zhou, DM]的文章
[Zou, HF]的文章
[Ni, JY]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。