中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Membrane affinity chromatography for analysis and purification of biopolymers
作者: Zhou, DM;  Zou, HF;  Ni, JY;  Wang, HL;  Yang, L;  Zhang, YK
关键词: column liquid chromatography ;  membrane stationary phase ;  immunoaffinity analysis ;  affinity efficiency ;  adsorption capacity
刊名: CHROMATOGRAPHIA
发表日期: 1999-07-01
卷: 50, 期:1-2, 页:27-34
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Affinity columns suitable for HPLC were prepared by immobilization of various ligands of protein A, human IgG, human IgM and pectinase on GMA modified cellulose membrane. The adsorption capacity, affinity efficiency and activity recovery of various IgGs on these affinity columns were measured. It was observed that the length of the coupling arm plays a very important role in affinity efficiency, and the effect of eluent flow-rate on adsorption capacity was very small. The protein A column was exploited for the process monitoring of dog IgG in clinical experiments on immuno-adsorption therapy. A pectinase column was used for the determination of polygalacturonase inhibiting proteins first purified on a hydroxyapatite column. It took only about 2.5 min for analysis at a flow-rate of 1.0 mL min(-1). The high speed analysis of biopolymers could be performed at a flow rate of 6.0 mL min(-1) within 15 s. Membrane affinity chromatography gives good reproducibility, high efficiency, low column-pressure and is rapid. It can also be used for micro-scale purification of biopolymers.
关键词[WOS]: BIOMOLECULES ;  PERFORMANCE ;  PROTEINS ;  REMOVAL
语种: 英语
WOS记录号: WOS:000081720200006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138576
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhou, DM,Zou, HF,Ni, JY,et al. Membrane affinity chromatography for analysis and purification of biopolymers[J]. CHROMATOGRAPHIA,1999,50(1-2):27-34.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhou, DM]'s Articles
 [Zou, HF]'s Articles
 [Ni, JY]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhou, DM]‘s Articles
 [Zou, HF]‘s Articles
 [Ni, JY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace