Membrane affinity chromatography for analysis and purification of biopolymers
Zhou, DM; Zou, HF; Ni, JY; Wang, HL; Yang, L; Zhang, YK
KeywordColumn Liquid Chromatography Membrane Stationary Phase Immunoaffinity Analysis Affinity Efficiency Adsorption Capacity
Indexed BySCI
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
AbstractAffinity columns suitable for HPLC were prepared by immobilization of various ligands of protein A, human IgG, human IgM and pectinase on GMA modified cellulose membrane. The adsorption capacity, affinity efficiency and activity recovery of various IgGs on these affinity columns were measured. It was observed that the length of the coupling arm plays a very important role in affinity efficiency, and the effect of eluent flow-rate on adsorption capacity was very small. The protein A column was exploited for the process monitoring of dog IgG in clinical experiments on immuno-adsorption therapy. A pectinase column was used for the determination of polygalacturonase inhibiting proteins first purified on a hydroxyapatite column. It took only about 2.5 min for analysis at a flow-rate of 1.0 mL min(-1). The high speed analysis of biopolymers could be performed at a flow rate of 6.0 mL min(-1) within 15 s. Membrane affinity chromatography gives good reproducibility, high efficiency, low column-pressure and is rapid. It can also be used for micro-scale purification of biopolymers.
WOS IDWOS:000081720200006
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Zhou, DM,Zou, HF,Ni, JY,et al. Membrane affinity chromatography for analysis and purification of biopolymers[J]. CHROMATOGRAPHIA,1999,50(1-2):27-34.
APA Zhou, DM,Zou, HF,Ni, JY,Wang, HL,Yang, L,&Zhang, YK.(1999).Membrane affinity chromatography for analysis and purification of biopolymers.CHROMATOGRAPHIA,50(1-2),27-34.
MLA Zhou, DM,et al."Membrane affinity chromatography for analysis and purification of biopolymers".CHROMATOGRAPHIA 50.1-2(1999):27-34.
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