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题名: High efficiency DNA separation by capillary electrophoresis in a polymer solution with ultralow viscosity
作者: Han, FT;  Huynh, BH;  Ma, YF;  Lin, BC
刊名: ANALYTICAL CHEMISTRY
发表日期: 1999-07-01
卷: 71, 期:13, 页:2385-2389
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The viscosities of some polymer solutions for DNA separation in capillary electrophoresis are generally very high, which makes them hard to pump into the capillaries. We have developed a novel sieving buffer, based on low-molecular-weight hydroxypropylmethylcellulose, to separate DNA fragments. The viscosity of this sieving matrix was at least I order of magnitude lower than that of traditional buffers with similar sieving effect, The influence of additives such as urea and mannitol was investigated. It was found that the double-stranded DNA (ds DNA) fragments began to denature in 3.5 M urea, and 7 M urea can denature the ds DNA completely. The presence of mannitol will decrease the overlap threshold of the polymer solution (the concentration at which the polymer molecules begin to entangle with each other), which makes it possible to separate DNA fragments in a polymer solution of relatively low concentration. The influence of the electrical held was also investigated, and it was found that the mobility of DNA fragments up to 2000 bp in length did not change greatly with different electric fields. This phenomenon implies that the DNA fragments at this range do not change their conformation with the increase of electric field as was previously believed. The possible mechanism for the separation of DNA fragments is also discussed.
关键词[WOS]: FRAGMENTS ;  SIZE
语种: 英语
WOS记录号: WOS:000081265600032
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138580
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Truman State Univ, Kirksville, MO 63501 USA
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Han, FT,Huynh, BH,Ma, YF,et al. High efficiency DNA separation by capillary electrophoresis in a polymer solution with ultralow viscosity[J]. ANALYTICAL CHEMISTRY,1999,71(13):2385-2389.
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