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题名: Investigation of molecular association by capillary zone electrophoresis frontal analysis
作者: Ding, YS;  Lin, BC
关键词: capillary electrophoresis ;  frontal analysis ;  propranolol ;  human serum albumin ;  association parameters
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 1999
卷: 27, 期:1, 页:20-23
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A capillary zone electrophoresis/Frontal Analysis method was discussed and applied to the study of association parameters of a basic bug, propranolol (PRO), to human serum albumin (HSA) under drug-HSA interaction equilibra. After the direct hydrodynamic injection (110 kPa. s) of a drug-HSA mixture into a coated capillary (effective length, 32 cm; 50 mu m i.d.), the basic bug is eluted as a zonal peak with a plateau region under condition of phosphate buffer ( pH 7.4, ionic strength = 0.17) at 10 kV running voltage. The unbound concentration of bug can be determined from the plateau peak height with good linear response, r = 0.9991 (n = 8). Eight PRO-HSA solutions (containing 100 similar to 450 mu mol/L of PRO and 400 similar to 50 mu mol/L, of HSA, respectively) were investigated. The obtained data were processed with Scatchard linear regression analysis and the final estimated PRO-HSA association parameters include binding sites n = 0.58 (+/- 0.03) and binding constant K = 1500(+/- 110).
关键词[WOS]: BINDING CONSTANTS ;  LIGANDS ;  DRUG
语种: 英语
WOS记录号: WOS:000080031800005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138598
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Ding, YS,Lin, BC. Investigation of molecular association by capillary zone electrophoresis frontal analysis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,1999,27(1):20-23.
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