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Investigation of molecular association by capillary zone electrophoresis frontal analysis
Ding, YS; Lin, BC
KeywordCapillary Electrophoresis Frontal Analysis Propranolol Human Serum Albumin Association Parameters
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
1999
Volume27Issue:1Pages:20-23
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordBINDING CONSTANTS ; LIGANDS ; DRUG
AbstractA capillary zone electrophoresis/Frontal Analysis method was discussed and applied to the study of association parameters of a basic bug, propranolol (PRO), to human serum albumin (HSA) under drug-HSA interaction equilibra. After the direct hydrodynamic injection (110 kPa. s) of a drug-HSA mixture into a coated capillary (effective length, 32 cm; 50 mu m i.d.), the basic bug is eluted as a zonal peak with a plateau region under condition of phosphate buffer ( pH 7.4, ionic strength = 0.17) at 10 kV running voltage. The unbound concentration of bug can be determined from the plateau peak height with good linear response, r = 0.9991 (n = 8). Eight PRO-HSA solutions (containing 100 similar to 450 mu mol/L of PRO and 400 similar to 50 mu mol/L, of HSA, respectively) were investigated. The obtained data were processed with Scatchard linear regression analysis and the final estimated PRO-HSA association parameters include binding sites n = 0.58 (+/- 0.03) and binding constant K = 1500(+/- 110).
Language英语
WOS IDWOS:000080031800005
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138598
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Ding, YS,Lin, BC. Investigation of molecular association by capillary zone electrophoresis frontal analysis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,1999,27(1):20-23.
APA Ding, YS,&Lin, BC.(1999).Investigation of molecular association by capillary zone electrophoresis frontal analysis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,27(1),20-23.
MLA Ding, YS,et al."Investigation of molecular association by capillary zone electrophoresis frontal analysis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 27.1(1999):20-23.
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