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Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase
Yang, L; Jia, LY; Zou, HF; Zhang, YK
刊名BIOMEDICAL CHROMATOGRAPHY
1999-05-01
13期:3页:229-234
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]ZINC CHELATE ; ADSORPTION ; PROTEINS ; ALBUMIN ; BINDING ; PHASES ; IMAC
英文摘要A metal ion chelating membrane medium based on iminodiacetate-substituted modified short cotton cellulose was examined for the purification of bovine liver catalase (BLC). The effect of buffer pH, chelator surface density, initial concentration of crude enzyme and flow rate on BLC binding efficiency to the copper ion chelating membrane adsorbent were examined. Under the chromatographic conditions chosen, 67.7% recovery of BLC was attained with an overall 4.2-fold increase in specific activity in a single step. After performance of BLC purification, the chelating membrane adsorbent can be easily regenerated by imidazole or EDTA buffer with higher reviving effectiveness with the latter. Copyright (C) 1999 John Wiley & Sons, Ltd.
语种英语
WOS记录号WOS:000080542100006
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被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/138636
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Yang, L,Jia, LY,Zou, HF,et al. Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(3):229-234.
APA Yang, L,Jia, LY,Zou, HF,&Zhang, YK.(1999).Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase.BIOMEDICAL CHROMATOGRAPHY,13(3),229-234.
MLA Yang, L,et al."Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase".BIOMEDICAL CHROMATOGRAPHY 13.3(1999):229-234.
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