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题名: Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase
作者: Yang, L;  Jia, LY;  Zou, HF;  Zhang, YK
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 1999-05-01
卷: 13, 期:3, 页:229-234
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Pharmacology & Pharmacy
英文摘要: A metal ion chelating membrane medium based on iminodiacetate-substituted modified short cotton cellulose was examined for the purification of bovine liver catalase (BLC). The effect of buffer pH, chelator surface density, initial concentration of crude enzyme and flow rate on BLC binding efficiency to the copper ion chelating membrane adsorbent were examined. Under the chromatographic conditions chosen, 67.7% recovery of BLC was attained with an overall 4.2-fold increase in specific activity in a single step. After performance of BLC purification, the chelating membrane adsorbent can be easily regenerated by imidazole or EDTA buffer with higher reviving effectiveness with the latter. Copyright (C) 1999 John Wiley & Sons, Ltd.
关键词[WOS]: ZINC CHELATE ;  ADSORPTION ;  PROTEINS ;  ALBUMIN ;  BINDING ;  PHASES ;  IMAC
语种: 英语
WOS记录号: WOS:000080542100006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138636
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Yang, L,Jia, LY,Zou, HF,et al. Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(3):229-234.
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