DICP OpenIR
Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase
Yang, L; Jia, LY; Zou, HF; Zhang, YK
Source PublicationBIOMEDICAL CHROMATOGRAPHY
1999-05-01
Volume13Issue:3Pages:229-234
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS KeywordZINC CHELATE ; ADSORPTION ; PROTEINS ; ALBUMIN ; BINDING ; PHASES ; IMAC
AbstractA metal ion chelating membrane medium based on iminodiacetate-substituted modified short cotton cellulose was examined for the purification of bovine liver catalase (BLC). The effect of buffer pH, chelator surface density, initial concentration of crude enzyme and flow rate on BLC binding efficiency to the copper ion chelating membrane adsorbent were examined. Under the chromatographic conditions chosen, 67.7% recovery of BLC was attained with an overall 4.2-fold increase in specific activity in a single step. After performance of BLC purification, the chelating membrane adsorbent can be easily regenerated by imidazole or EDTA buffer with higher reviving effectiveness with the latter. Copyright (C) 1999 John Wiley & Sons, Ltd.
Language英语
WOS IDWOS:000080542100006
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/138636
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Yang, L,Jia, LY,Zou, HF,et al. Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase[J]. BIOMEDICAL CHROMATOGRAPHY,1999,13(3):229-234.
APA Yang, L,Jia, LY,Zou, HF,&Zhang, YK.(1999).Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase.BIOMEDICAL CHROMATOGRAPHY,13(3),229-234.
MLA Yang, L,et al."Immobilized iminodiacetic acid (IDA)-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase".BIOMEDICAL CHROMATOGRAPHY 13.3(1999):229-234.
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