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题名: Determination of ginsenoside Rg3 in plasma by solid-phase extraction and high-performance liquid chromatography for pharmacokinetic study
作者: Wang, HL;  Zou, HF;  Kong, L;  Zhang, YK;  Pang, H;  Su, CY;  Liu, GY;  Hui, M;  Fu, L
关键词: ginsenosides
刊名: JOURNAL OF CHROMATOGRAPHY B
发表日期: 1999-08-20
卷: 731, 期:2, 页:403-409
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A method using high-performance liquid chromatography (HPLC) and solid-phase extraction (SPE) is described for the determination of ginsenoside Rg3 in human plasma. A 2.5-ml volume of plasma was mixed with 2.5 mi 60% methanol aqueous solution, and centrifuged at 1100 g for 10 min, the supernatant fluid was further purified by SPE with 200 mg/5 mi 40 mu m octadecyl silica and separation was obtained using a reversed-phase column under isocratic conditions with ultraviolet absorbance detection. The intra- and inter-day precision, determined as relative standard deviations, were less than 5.0%, and method recovery was more than 97%. The lower limit of quantitation, based on standards with acceptable RSDs, was 2.5 ng/ml. No endogenous compounds were found to interfere with analyte. A good linear relationship with a regression coefficient of 0.9999 in the range of 2.5 to 200 ng/ml was observed. This method has been demonstrated to be suitable for pharmacokinetic studies in humans. Method development for determination of drug with low UV absorption by SPE and HPLC is also discussed. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
关键词[WOS]: ACIDIC SAPONINS ;  POROUS-GLASS
语种: 英语
WOS记录号: WOS:000082472800028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/138639
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr China, Dalian 116011, Peoples R China
2.Dalian Med Univ, Dept Clin Pharmacol, Dalian 116023, Peoples R China
3.Dalian Tianfu Inst Nat Med, Dalian 116600, Peoples R China

Recommended Citation:
Wang, HL,Zou, HF,Kong, L,et al. Determination of ginsenoside Rg3 in plasma by solid-phase extraction and high-performance liquid chromatography for pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B,1999,731(2):403-409.
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